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Series GSE35841 Query DataSets for GSE35841
Status Public on Feb 16, 2012
Title Derivation of rat induced pluripotent stem cells that share similarities with embryonic stem cells using a transposon system
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Induced pluripotent stem (IPS) cells have attracted enormous attention due to their vast potential in regenerative medicine, pharmaceutical screening and basic research. The majority of prior established rat IPS cells were generated from somatic cells by retroviral and lentiviral transduction with expression of Oct4, Sox2, Klf4 and c-Myc and using chemical inhibitors of key differentiation pathways. A major difficulty in the application of this technology is the efficient delivery of reprogramming factors and the long-term maintenance of properties of stem cells.

Here, we employed the PiggyBac (PB) transposon carrying four 2A peptide-linked reprogramming factors for generating rat IPS cells. These stable rat IPS cells are similar to embryonic stem (ES) cells in morphology, proliferation, teratoma formation, expression characteristic pluripotency markers, developmental potential, and germline transmission. Transcriptional profiling of the IPS cells revealed both pathways in common with ES cells from rat and unique signaling pathway to our cells, including Wnt, TGF and Notch. The cell lines and information obtained in this study will accelerate our understanding of the molecular regulation underlying germline pluripotency and pave the way for exploration of cell-based therapies using the rat.
 
Overall design To compare the gene expression profiling between rat IPS cells and ES cells to show if the rat IPS cells had been reprogrammed into pluripotent status like rat ES cells at the gene expression level.
 
Contributor(s) Yang S, Schilling R, Foeckler J, Hessner M, Jia S, Zappa A, Takizawa A, Kalloway S, Geurts AM, Jacob HJ
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Submission date Feb 15, 2012
Last update date Jul 31, 2017
Contact name Sheng Yang
E-mail(s) syang@mcw.edu
Organization name Medical College of Wisconsin
Department Human and Molecular Genetics Center
Lab Jacob and Geurt
Street address 8701 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 52336
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (12)
GSM876103 AG9 FHH-IPS-A1-rep1
GSM876104 AG10 FHH-IPS-A1-rep2
GSM876105 AG11 FHH-IPS-A2-rep1
Relations
BioProject PRJNA151851

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35841_RAW.tar 28.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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