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Series GSE35889 Query DataSets for GSE35889
Status Public on Feb 18, 2012
Title Expression data from adult wild-type and zCdx1b transgenic zebrafish (3 months old)
Organism Danio rerio
Experiment type Expression profiling by array
Summary Cdx2 has been suggested to play an important role in Barrett's esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, in vivo data have been lacking. The aim of the present study was to investigate whether transgenic overexpression of zCdx1b, the functional equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish
A transgenic zebrafish system was developed by expressing zCdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). zCdx1b expression in the esophageal squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization.
Overall design The upper digestive tract tissue from 3 adult zebrafish (3 months old) was pooled as one sample. Three zCdx1b transgenic samples were used for microarray, and 2 wild type samples were used for control.
Contributor(s) Hu B, Chen X
Citation(s) 23672288
Submission date Feb 16, 2012
Last update date Oct 16, 2018
Contact name Hao Chen
Organization name North Carolina Central University
Department BBRI
Lab Xiaoxin Luke Chen's Lab
Street address 700 George Street
City Durham
ZIP/Postal code 27707
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (5)
GSM877078 zCdx1b transgenic zebrafish, biological rep1
GSM877079 zCdx1b transgenic zebrafish, biological rep2
GSM877080 zCdx1b transgenic zebrafish, biological rep3
BioProject PRJNA151947

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35889_RAW.tar 8.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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