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| Status |
Public on Feb 22, 2012 |
| Title |
miRNA Expression Profiling in Ovarian Cell Lines compared to pooled commercial RNA samples |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We investigated whether levels of serum microRNAs (miRNAs) could discriminate women with high-grade serous ovarian epithelial cancer (SEOC) from age matched healthy volunteers.
miRNA expression profiling was performed on 4 SEOC cell lines and normal human ovarian surface epithelial cells (HOSE). miR-182, miR-200a, b and c were highly overexpressed in the SEOC cell lines. miR-103, miR-92a and miR-638 displayed relatively invariant expression and with RNU48, were assessed, as putative serum miRNA normalizers. The 7 miRNA and RNU48 were assessed in serum from SEOC patients (n = 30) and age-matched healthy volunteers (n = 32) by qRT-PCR following pre-amplification.
No correlation between age and miRNA or RNU48 levels was observed (age range 30-79 years). miR-103 demonstrated the least variance and was chosen to normalize serum volume adjusted results. miR-200a, b and c were significantly elevated in SEOC serum (P = 0.002; 0.003; 0.0003 respectively) and a multivariate model was the best predictive classifier of SEOC (ROC-AUC = 0.806). A correlation between high miR-200b levels and residual macroscopic disease following cytoreductive surgery was identified (P = 0.006).
Our results suggest that serum miR-200a, b and c may have utility as diagnostic biomarkers for SEOC.
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| Overall design |
miRNA expression was arrayed in 1 human surface epithelial cells (HOSE) and 4 ovarian cancer cell lines (OV167, OV202, OVCAR-3, PE01). HOSE cells were run in triplicate (including 1 dye-swap). Ovarian cancer cell lines were run in duplicate (including 1 dye-swap).
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| Contributor(s) |
Kan CW, Hahn MA, Huh J, Gard G, Maidens J, Marsh DJ, Howell VM |
| Citation(s) |
23272653, 24737690 |
| Submission date |
Feb 21, 2012 |
| Last update date |
Sep 18, 2014 |
| Contact name |
Casina W.S Kan |
| E-mail(s) |
casina.kan@sydney.edu.au
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| Organization name |
Kolling Institute of Medical Research
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| Department |
Hormones and Cancer Group
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| Lab |
Functional Genomics Lab
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| Street address |
RNSH, Pacific Hwy
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| City |
St Leonards, Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2065 |
| Country |
Australia |
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| Platforms (1) |
| GPL15257 |
Exiqon MiRCURY Locked Nucleic Acid array [miRBase 8.2] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA151985 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35951_Exiqon_v8.1_print_2.gal.gz |
26.7 Kb |
(ftp)(http) |
GAL |
| GSE35951_RAW.tar |
3.6 Mb |
(http)(custom) |
TAR (of SPOT) |
| Processed data included within Sample table |
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