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| Status |
Public on Nov 12, 2012 |
| Title |
High-throughput sequencing of small RNAs (25-70 nt) from Drosophila wild-type and Dnmt2 mutants |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Dnmt2 genes are highly conserved tRNA methyltransferases with biological roles in cellular stress responses. The absence of obvious mutant phenotypes under laboratory conditions suggested a function for Dnmt2 under non-physiological conditions. Indeed, Dnmt2 has recently been implicated in various aspects of the cellular stress response and the tRNA methyltransferase activity of Dnmt2 has been shown to interfere with stress-induced fragmentation of various tRNAs. We used adult animals and small RNA sequencing during a heat stress experiment to determine the tRNA fragment abundance and identities in wild-type and Dnmt2 mutant somatic tissues. Dnmt2 mutants produced tRNA fragments with different identities when compared to wild-type controls, indicating the accumulation of non-physiological tRNA-derived molecules in tissues without Dnmt2.
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| Overall design |
6 samples examined: heterozygous and Dnmt2 mutant under control, heat shock and recovery conditions
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| Contributor(s) |
Durdevic Z, Schaefer M |
| Citation(s) |
24012760 |
| Submission date |
Feb 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Schaefer Matthias |
| E-mail(s) |
matthias.schaefer@meduniwien.ac.at
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| Organization name |
Medical University Vienna, Center for Anatomy & Cell Biology
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| Department |
Cell and Developmental Biology
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| Lab |
Schaefer Lab
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| Street address |
Schwarzspanierstrasse 17-I
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| City |
Vienna |
| State/province |
AT |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| GSM878560 |
endogenous small RNAs from heterozygous; control, 10 day old, male, whole body minus testis |
| GSM878561 |
endogenous small RNAs from heterozygous; heat shock, 10 day old, male, whole body minus testis |
| GSM878562 |
endogenous small RNAs from heterozygous; 2 day recovery, 12 day old, male, whole body minus testis |
| GSM878563 |
endogenous small RNAs from Dnmt2 mutants; control, 10 day old, male, whole body minus testis |
| GSM878564 |
endogenous small RNAs from Dnmt2 mutants; heat shock, 10 day old, male, whole body minus testis |
| GSM878565 |
endogenous small RNAs from Dnmt2 mutants; 2 day recovery, 12 day old, male, whole body minus testis |
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| Relations |
| SRA |
SRP011006 |
| BioProject |
PRJNA151637 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35981_RAW.tar |
250.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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