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| Status |
Public on Feb 19, 2015 |
| Title |
Lentiviral vector-based insertional mutagenesis identifies new clinically relevant candidate cancer genes involved in the pathogenesis of hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We devised a novel insertional mutagenesis approach based on lentiviral vectors to induce hepatocellular carcinoma in three mouse models and identified four novel cancer initiating genes. Two genes are the well characterized Braf and Sos1, while the other two are Fign, encoding an AAA ATPase whose functions are poorly understood, and the paternally expressed gene Rtl1 within the complex Dlk1-Dio3 imprinted region recently involved in stemness. Interestingly, Fign and Braf regulate the expression of the maternal genes of the Dlk1-Dio3 imprinted region, suggesting that both maternally and paternally expressed genes of this region play a role in hepatocarcinogenesis. Moreover, all the genes identified are upregulated and/or amplified/deleted in human hepatocellular carcinoma and play a relevant role in human hepatocarcinogenesis, as their expression levels and/or transcriptional signatures induced by their deregulation predict a different clinical outcome in hepatocellular carcinoma patients.
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| Overall design |
Primary human hepatocytes were transduced with SINLV.ET.trBRAF, SINLV.PGK.GFP or mock treated. RNA was collected at 5 different timepoints post-transduction: 24h, 36h, 48h, 72h, 144h (6d). Each experimental point was done in triplicate (A,B,C)
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| Contributor(s) |
Ranzani M, Cesana D, Bartholomä C, Sanvito F, Pala M, Benedicenti F, Riba M, Gallina P, Sergi LS, Merella S, Bulfone A, Doglioni C, Stupka E, Von Kalle C, Kim YJ, Schmidt M, Tonon G, Naldini L, Montini E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 21, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Marco Ranzani |
| E-mail(s) |
ranzani.marco@hsr.it
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| Organization name |
HSR-TIGET
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| Department |
TIGET
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| Lab |
Montini
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| Street address |
via olgettina 58
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| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA151647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35986_RAW.tar |
199.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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