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| Status |
Public on Jul 31, 2012 |
| Title |
Global changes in gene expression in primary keratinocytes after KLF9 overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify putative target genes of the transcription factor Klf9 in primary keratinocytes we over-expressed Klf9 in these cells using a lentiviral delivery system. We detected several hundred genes that show differential expression levels following ectopic Klf9 expression including several genes that are involved in proliferation and cell cycle control. These results allow insights into the mechanisms by which Klf9 regulates proliferation in primary keratinocytes.
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| Overall design |
Primary human neonatal foreskin keratinocytes were infected with equal viral titers of a GFP and KLF9 expression construct, respectively. Cells were harvested 2,4 and 7 days after infection and total RNA was used to perform whole genome microarray analysis.
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| Contributor(s) |
Spoerl F |
| Citation(s) |
22711835 |
| Submission date |
Feb 22, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Florian Spoerl |
| E-mail(s) |
Florian.Spoerl@gmx.de
|
| Organization name |
Beiersdorf AG
|
| Department |
Aged Skin
|
| Lab |
Dry Aged Skin
|
| Street address |
Unnastr. 48
|
| City |
Hamburg |
| ZIP/Postal code |
20245 |
| Country |
Germany |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA151719 |
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