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Series GSE36083 Query DataSets for GSE36083
Status Public on Feb 27, 2012
Title Transcriptomic Analysis of Placenta Affected by Antiphospholipid Antibodies: Following the TRAIL of trophoblast death
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Antiphospholipid antibodies, a maternal risk factor for preeclampsia, increase shedding of necrotic trophoblast debris from the placenta, leading to endothelial dysfunction. Using Affymetrix HGU133 Plus 2 microarrays, we found changes in the transcriptome of placental explants treated with antiphospholipid antibodies including seven mRNAs encoding for genes BCL2L1, MCL1, PDCD2L, FASLG, SEMA6A, PRKCE and TRAIL that are involved in the regulation of apoptosis. Quantitative real-time RT-PCR and immunohistochemistry confirmed a reduction in TRAIL expression. These results may help to understand how antiphospholipid antibodies affect trophoblast cell death and how the antibodies could contribute to the pathogenesis of preeclampsia.
 
Overall design Three different first-trimester placentas (8-8.5 weeks gestation) were dissected into 6 explants each and cultured either with antiphospholipid antibodies (25ug/mL) or untreated media for 16h. RNA was extracted from treated placental explants and Affymetrix HGU133 Plus 2 arrays were conducted to investigate changes in mRNA expression.

Untreated: 1UNT, 2UNT, 3UNT
Antiphospholipid antibody treatment: 1APL, 2APL, 3APL
Biological replicates: 1, 2, 3
 
Contributor(s) Pantham P, Rosario R, Chen Q, Print CG, Chamley LW
Citation(s) 22542907
Submission date Feb 26, 2012
Last update date Mar 25, 2019
Contact name Priyadarshini Pantham
E-mail(s) p.pantham@auckland.ac.nz
Phone 0064 9 373 7599
Fax 0064 9 373 7599
Organization name The University of Auckland
Department Obstetrics & Gynaecology
Lab Reproductive Biology & Immunology
Street address 85 Park Road
City Auckland
ZIP/Postal code 1023
Country New Zealand
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM880866 plac_unt_1
GSM880867 plac_apl_1
GSM880868 plac_unt_2
Relations
BioProject PRJNA151733

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36083_Annotations_U133plus2_2010-03-03_.txt.gz 972.8 Kb (ftp)(http) TXT
GSE36083_RAW.tar 27.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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