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Series GSE36089 Query DataSets for GSE36089
Status Public on Oct 18, 2012
Title Inflammatory mediators alter astrocyte genomic profiles and G-protein receptor evoked calcium signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inflammation features in diverse central nervous system disorders such as stroke, trauma, neurodegeneration, infection and autoimmunity. To better understand how inflammatory mediators may alter astrocyte functions, we examined the effects of transforming growth factor-β1 (TGF-β1), lipopolysaccharide (LPS) and interferon-gamma (IFNγ) on purified, murine, primary cortical astrocyte cultures. We used microarrays to conduct whole genome expression profiling, and measured calcium signaling, which is implicated in mediating dynamic astrocyte functions. Combinatorial exposure to TGF-β1, LPS and IFNγ significantly modulated astrocyte expression of over 6,800 genes and resulted in both additive and synergistic changes compared with individual stimuli alone. Bioinformatic analysis revealed that combinatorial treatment significantly and markedly up regulated molecular networks and pathways associated with immune signaling and with regulation of cell compromise, death, growth and proliferation. These findings provide databases of astrocyte transcriptome changes elicited by the inflammatory stimuli, TGF-β1, LPS and IFNγ alone and in combination, and show that these stimuli up regulate astrocyte molecular networks associated with immune- and injury-related functions and significantly alter astrocyte calcium signaling evoked by multiple GPCR.
 
Overall design We used microarrays to examine the effects of transforming growth factor-β1 (TGF-β1), lipopolysaccharide (LPS) and interferon-gamma (IFNγ) on purified, murine, primary cortical astrocyte cultures.
 
Contributor(s) Hamby ME, Coppola G, Geschwind DH, Khakh BS, Sofroniew MV
Citation(s) 23077035, 24106265
Submission date Feb 27, 2012
Last update date Jun 14, 2018
Contact name Giovanni Coppola
E-mail(s) gcoppola@ucla.edu
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM880929 LPS_IFNg_1
GSM880930 BASAL_1
GSM880931 TGFb1_LPS_IFNg_1
Relations
BioProject PRJNA151907

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36089_RAW.tar 3.1 Mb (http)(custom) TAR
GSE36089_non-normalized.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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