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Series GSE36322 Query DataSets for GSE36322
Status Public on Jul 06, 2012
Title Gene expression analysis of pluripotent and differentiated genes during EB formation treated with streptozotocin, o-glacnacylation inhibitor
Organism Mus musculus
Experiment type Expression profiling by array
Summary O-linked-N-acetylglucosamine (O-GlcNAc) has emerged as a critical regulator of diverse cellular processes, but its role in embryonic stem cells (ESCs) and pluripotency has not been investigated. Here we show that O-GlcNAcylation directly regulates core components of the pluripotency network. Blocking O-GlcNAcylation disrupts ESC self-renewal and reprogramming of somatic cells to induced pluripotent stem cells. The core reprogramming factors Oct4 and Sox2 are O-GlcNAcylated in ESCs, but the O-GlcNAc modification is rapidly removed upon differentiation. O-GlcNAc modification of Threonine 228 in Oct4 regulates Oct4 transcriptional activity and is important for inducing many pluripotency related genes, including Klf2, Klf5, Nr5a2, Tbx3 and Tcl1. A T228A point mutation that eliminates this O-GlcNAc modification reduces the capacity of Oct4 to maintain ESC self-renewal and reprogram somatic cells. Overall, our study makes a direct connection between O-GlcNAcylation of key regulatory transcription factors and the activity of the pluripotency network.
 
Overall design 2 of E14 stem cell, 2 of embryonic body at day 2, 2 of embryonic body at day 2 treated with streptozotocin, 1 of ZHBTc4 stem cell treated with doxicyclin, and 1 of ZHBTc4 stem cell treated with doxicyclin and streptozotocin were analysed
 
Contributor(s) Youn H, Jang H, Kim TW
Citation(s) 22608532
Submission date Mar 07, 2012
Last update date Jun 14, 2018
Contact name Taewan Kim
E-mail(s) taewan79@snu.ac.kr
Phone 082-2-3668-7629
Organization name Seoul National University of medicine
Department biomedical science
Lab metabolic checkpoint lab
Street address biomedical science building 202ho
City Youngun dong, jongro gu,Seoul
ZIP/Postal code 110-799
Country South Korea
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM888897 E14 stem cell, undiffrentiated state, rep 1
GSM888898 E14 stem cell, undiffrentiated state, rep 2
GSM888899 EB2, embryonic body formation for 2 days, rep 1
Relations
BioProject PRJNA153369

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36322_RAW.tar 3.1 Mb (http)(custom) TAR
GSE36322_non-normalized_data.txt.gz 12.8 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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