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Series GSE36377 Query DataSets for GSE36377
Status Public on Nov 05, 2012
Title Activating transcription factor 3 regulates immune and metabolic homeostasis
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Integration of metabolic, stress and immune responses plays a fundamental role during animal development to maintain energy homeostasis while ensuring growth and proper developmental timing. Perturbation of metabolic and immune signaling circuits has detrimental consequences to animal development including growth retardation, organ malfunction and emergence of the metabolic syndrome. Here, we demonstrate that the Drosophila basic region-leucine zipper (bZIP) protein, Activating transcription factor 3 (Atf3), safeguards a balance of metabolic and immune system responses during fly development. Loss of Atf3 function results in lethality during late-larval and pupal stages. Atf3-deficient larvae exhibit phenotypes resembling the metabolic syndrome in mammals. Excessive accumulation of lipids in the larval fat body and gut is accompanied by altered expression of genes involved in lipid metabolism. Moreover, the fat body of atf3 mutants becomes infiltrated by hemocytes. The major pro-inflammatory pathways signaling through JNK and Imd are hyperactivated in atf3 mutants, causing ectopic expression of antimicrobial peptide genes. Suppression of the immune response, achieved by reducing the gene dose of the transcription factors FOXO or NF-kappaB/Relish, significantly improves lipid metabolism and normalizes gene expression profile of atf3 mutants. In addition, heterozygosity of relish partially rescues lethality of the atf3 mutants. Our data thus identify Atf3 as an essential player that links metabolic and immune system homeostasis during animal development.
 
Overall design Examination of mRNA levels from four genotypes of male, 3rd instar Drosophila melanogaster larvae. mRNA levels from four genotypes relative to y w control were determined using two biological replicates per genotype.
Genome build: BDGP R5/dm3, April 2006
 
Contributor(s) Rynes J, Donohoe C, Frommolt P, Brodesser S, Jindra M, Uhlirova M
Citation(s) 22851689
Submission date Mar 08, 2012
Last update date May 15, 2019
Contact name Mirka Uhlirova
E-mail(s) mirka.uhlirova@uni-koeln.de
Organization name CECAD, University of Cologne
Department Institute For Genetics
Street address Zülpicher Str. 47a
City Cologne
ZIP/Postal code 50674
Country Germany
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (10)
GSM890561 YW_SAMPLE1
GSM890562 YW_SAMPLE2
GSM890563 ATF3[76]_SAMPLE1
Relations
SRA SRP011390
BioProject PRJNA153257

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36377_ATF3_76_PROCESSED.txt.gz 421.4 Kb (ftp)(http) TXT
GSE36377_ATF3_A_PROCESSED.txt.gz 424.6 Kb (ftp)(http) TXT
GSE36377_FOXO_PROCESSED.txt.gz 426.7 Kb (ftp)(http) TXT
GSE36377_REL_PROCESSED.txt.gz 428.2 Kb (ftp)(http) TXT
GSE36377_ReadMe_Processed_Data.txt 1.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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