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Series GSE36379 Query DataSets for GSE36379
Status Public on Mar 09, 2012
Title Expression data from mouse pancreatic cell lines treated with chromatin-targeting small molecules
Organism Mus musculus
Experiment type Expression profiling by array
Summary We measured the genome-wide expression changes induced by 29 compounds targeting HDACs, DNMTs, histone lysine methyltransferases (HKMTs), and protein arginine methyltransferases (PRMTs) in pancreatic α- and β-cell lines.
We used microarrays to detail the global programme of gene-expression changes induced by chromatin-targeting compounds at 1, 6, and 24 hours.
 
Overall design To identify a comprehensive list of target genes that can be regulated by modulating the activities of chromatin-modifying enzymes, we measured the genome-wide transcriptional effects of 29 compounds in pancreatic alpha and beta cell lines at three time points. The results indicate that compounds cause similar effects independent of the cell line in which they were profiled. All clinical HDAC inhibitors fell into the structural classes of hydroxamic acids and ortho-amino anilides, respectively and up- and down-regulated hundreds of transcripts. In contrast, more selective compounds like the HKMT inhibitor BIX-01294 have specific effects.
 
Contributor(s) Kubicek S, Gilbert JC, Fomina-Yadlin D, Gitlin AD, Yuan Y, Wagner FF, Holson EB, Luo T, Lewis TA, Taylor B, Gupta S, Shamji AF, Wagner BK, Clemons PA, Schreiber SL
Citation(s) 22434908, 24759943
Submission date Mar 08, 2012
Last update date Jul 29, 2019
Contact name Paul A Clemons
E-mail(s) pclemons@broadinstitute.org
Phone 617-714-7346
Organization name Broad Institute of Harvard and MIT
Department Chemical Biology Program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02143
Country USA
 
Platforms (1)
GPL8759 [HT_MG-430A] Affymetrix HT Mouse Genome 430A Array
Samples (594)
GSM890591 alpha-TC1 cells, 01-hr treatment, 4.3uM, BRD-K17311666, Replicate1, Batch_04, HTA6_D03
GSM890592 alpha-TC1 cells, 01-hr treatment, 4.3uM, BRD-K17311666, Replicate2, Batch_09, HTA9_G09
GSM890593 alpha-TC1 cells, 01-hr treatment, 4.3uM, BRD-K17311666, Replicate3, Batch_08, HTA3_B11
Relations
BioProject PRJNA153261

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36379_RAW.tar 1.3 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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