|
| Status |
Public on Jul 01, 2016 |
| Title |
Transcription profile of fetal human CD140a-defined OPC differentiation in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human fetal dissociates from 19-22 week gestational age were magnetically sorted for CD140a antigen. CD140a-defined OPCs were plated into serum free conditions and allowed to differentiate in the absence of growth factors or mitogens. RNA was extracted from cells immediately following isolation and every day for 4 days. To block differentiation, matched cells were cultured in the presence of PDGF-AA (10ng/ml). This treatment prevents the acquisition of O4-positive oligodendrocyte cell fate and delays MBP mRNA expression by human CD140a-sorted OPCs.
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| |
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| Overall design |
29 samples, 4 time points, 2 media conditions, at least three biological replicates per time point and media condition
|
| |
|
| Contributor(s) |
Pol SU, O'Bara MA, Vedia BH, Sim FJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 12, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Fraser James Sim |
| E-mail(s) |
fjsim@buffalo.edu
|
| Phone |
7168292151
|
| Organization name |
University at Buffalo
|
| Department |
Pharmacology
|
| Street address |
3435 Main Street
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14216 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (29)
|
|
| Relations |
| BioProject |
PRJNA153239 |
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