|
| Status |
Public on Jun 01, 2012 |
| Title |
Expression data from region-specific postnatal astrocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Astroglial cells in the adult brain constitute a heterogeneous population endowed with region-specific properties. Recently, they have acquired greater relevance as active components of the adult neural stem cell (aNSC) niches. Astrocytes located in the vicinity of aNSC reservoirs are thought to regulate aNSC behaviour. We have compared the function of glial cells isolated from the postnatal and adult subventricular zone and hippocampus (two stem cell niches, where aNSCs self-renew and give rise to immature neurons), from the olfactory bulb (a neurogenic region where the immature neurons cease to proliferate and terminally differentiate) and from a non-stem and non-neurogenic area such as the ventral mesencephalon. Co-culture experiments demonstrate that subventricular zone glial cells secrete soluble signals that promote NSC self-renewing divisions.
We used microarrays to detail the global gene expression of astroglial cells isolated from four different brain regions (olfactory bulb, ventral mesencephalon, hippocampus and subventricular zone) and identified up-regulated genes coding for secreted proteins in astrocytes from the subventricular zone.
|
| |
|
| Overall design |
Primary astrocytes were cultured from four CD-1 mouse brain regions and cells were employed for RNA extraction and hybridization on Affymetrix microarrays. Primary tissue for the astrocyte cultures was dissected from four postnatal day 3 littermate pups. The tissue from the three pups was pooled in order to reduce individual differences of expression profiles.
|
| |
|
| Contributor(s) |
Mireia M, Helena M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 13, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Helena Mira |
| E-mail(s) |
hmira@isciii.es
|
| Organization name |
Instituto de Salud carlos III
|
| Department |
Área de Biología Celular y Desarrollo
|
| Lab |
Molecular Neurobiology Lab
|
| Street address |
Ctra. Majadahonda-Pozuelo, km 2.2
|
| City |
Majadahonda |
| State/province |
Madrid |
| ZIP/Postal code |
28220 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (4)
|
| GSM894111 |
primary astrocytes from the subventricular zone , biological rep1 |
| GSM894112 |
primary astrocytes from the ventral mesencephalon, biological rep1 |
| GSM894113 |
primary astrocytes from the olfactory bulb, biological rep1 |
| GSM894114 |
primary astrocytes from the hippocampus, biological rep1 |
|
| Relations |
| BioProject |
PRJNA153553 |
Less...
More...