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Series GSE36587 Query DataSets for GSE36587
Status Public on Nov 12, 2013
Title Transcriptional events during lung recovery from MRSA infection: a mouse pneumonia model
Organism Mus musculus
Experiment type Expression profiling by array
Summary Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging threat to human health throughout the world. Rodent MRSA pneumonia models mainly focus on the early innate immune responses to MRSA infection. However, the molecular pattern and mechanisms of recovery from MRSA lung infection are largely unknown. In this study, a nonlethal mouse MRSA pneumonia model was employed to investigate events during lung recovery from MRSA infection. We compared lung bacterial clearance, bronchoalveolar lavage fluid (BALF) characterization, lung histology, and gene expression profiling between Day 1 and Day 3 post-MRSA infection. Compared to Day 1 post-infection, bacterial colony counts and both BALF total cell number and protein concentration significantly decreased at Day 3 post-infection. Lung cDNA microarray analysis identified 47 significantly up-regulated and 35 down-regulated genes (p<0.01, 1.5-fold change [up and down]). Changes in eight genes were confirmed by real-time PCR. The pattern of gene expression suggests lung recovery is characterized by enhanced cell division, vascularization, and wound healing and by adjustment in host adaptive immune responses. Collectively, this data helps elucidate the molecular mechanisms of lung recovery after MRSA infection.
 
Overall design RNA expression analysis was performed using the Illumina MouseRef-8 v2.0 BeadChip (Illumina, San Diego, CA), which provides coverage of approximately 25,700 genes and expressed sequence tags. Four independent mouse lung tissue samples at Day 1 and Day 3 post-MRSA infection were used in this study.
 
Contributor(s) Chen J, Wunderink R, Feng G, Lin S
Citation(s) 23936388, 23826353
Submission date Mar 17, 2012
Last update date Aug 23, 2019
Contact name Jiwang Chen
E-mail(s) chenjw@uic.edu
Organization name University of Illinois at Chicago
Street address 840 S Wood Street
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM841761 Day 1 post-MRSA wild type infection, sample 1
GSM841762 Day 1 post-MRSA wild type infection, sample 2
GSM841763 Day 1 post-MRSA wild type infection, sample 3
Relations
BioProject PRJNA189790

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36587_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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