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| Status |
Public on Mar 21, 2012 |
| Title |
Aberrant promoter methylation and expression of UTF1 during cervix carcinogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern of stem cell (SC) markers during tumor development. In this study, we were interested to identify SC genes methylation profiles during cervical carcinogenesis. A genome-wide promoter methylation screening revealed a strong hypermethylation of Undifferentiated cell Transcription Factor 1 (UTF1) promoter in cervical cancer in comparison with normal ectocervix. By direct bisulfite pyrosequencing of DNA isolated from liquid-based cytological samples, we showed that UTF1 promoter methylation increases with lesion severity, the highest level of methylation being found in carcinoma. This hypermethylation was associated with increased UTF1 mRNA and protein expression. By using quantitative RT-PCR and Western Blot, we showed that both UTF1 mRNA and protein are present in epithelial cancer cell lines, even in the absence of its two main described regulators Oct4A and Sox2. Moreover, by immunofluorescence, we confirmed the nuclear localisation of UTF1 in cell lines. Surprisingly, direct bisulfite pyrosequencing revealed that the inhibition of DNA methyltransferase by 5-aza-2'-deoxycytidine was associated with decreased UTF1 gene methylation and expression in two cervical cancer cell lines of the four tested. These findings strongly suggest that UTF1 promoter methylation profile might be a useful biomarker for cervical cancer diagnosis and raise the questions of its role during epithelial carcinogenesis and of the mechanisms regulating its expression.
*Mouallif M and Guenin S contributed equally to this work
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| Overall design |
Bisulfite converted DNA from the 9 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
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| Contributor(s) |
Mouallif* M, Guenin* S, Lampe X, Krusy N, Delbecque K, Kridelka F, Fuks F, Ennaji M, Delvenne P |
| Citation(s) |
22880087 |
| Submission date |
Mar 20, 2012 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Samuel GUENIN |
| E-mail(s) |
samuel.guenin@gmail.com
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| Organization name |
Université de Liège
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| Department |
GIGA CANCER
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| Lab |
Experimental Pathology
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| Street address |
CHU Sart Tilman, B23+4
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| City |
LIEGE |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (9)
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| GSM897790 |
genomic DNA from frozen normal ectocervix 1 |
| GSM897791 |
genomic DNA from frozen normal ectocervix 2 |
| GSM897792 |
genomic DNA from frozen normal ectocervix 3 |
| GSM897793 |
genomic DNA from frozen normal ectocervix 4 |
| GSM897794 |
genomic DNA from frozen squamous carcinoma 1 |
| GSM897795 |
genomic DNA from frozen squamous carcinoma 2 |
| GSM897796 |
genomic DNA from frozen squamous carcinoma 3 |
| GSM897797 |
genomic DNA from frozen squamous carcinoma 4 |
| GSM897798 |
genomic DNA from frozen squamous carcinoma 5 |
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| Relations |
| BioProject |
PRJNA153637 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36637_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR |
| GSE36637_non-normalized.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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