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| Status |
Public on Jan 26, 2015 |
| Title |
Transcriptome profiling of trisomy 21 and euploid iPSC-derived hematopoietic progenitors expressing wtGATA1 or an amino-truncated isoform of GATA1, GATA1short (GATA1s). |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We generated human induced pluripotent stem cells (iPSCs) from trisomy 21 (T21) and euploid patient tissues with and without GATA1 mutations causing exclusive expression of truncated GATA1, termed GATA1short (GATA1s). Transcriptome analysis comparing expression levels of genes in GATA1s vs. wtGATA1-expressing progenitors demonstrated that GATA1s impairs erythropoiesis and enhances megakaryopoiesis and myelopoiesis in both T21 and euploid contexts in the iPSC-model system.
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| Overall design |
We analyzed the transcriptome of (i) T21 iPSC-derived heamtopoietic progenitors expressing wtGATA1 (6 replicates) or GATA1s (3 replicates), as well as of (ii) euploid iPSC-derived progenitors expressing wtGATA1 (2 replicates) or GATA1s (2 replicates).
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| Contributor(s) |
Byrska-Bishop M, VanDorn D, Campbell A, Betensky M, Arca PM, Yao Y, Gadue P, Costa FF, Nemiroff RL, Blobel GA, French DL, Hardison RC, Weiss MJ, Chou ST |
| Citation(s) |
25621499 |
| Submission date |
Mar 25, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Ross Hardison |
| E-mail(s) |
rch8@psu.edu
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| Organization name |
Pennsylvania State University
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| Street address |
303 Wartik Lab
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (13)
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| GSM901041 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep1 |
| GSM901042 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep2 |
| GSM901043 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep3 |
| GSM901044 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep4 |
| GSM901045 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep5 |
| GSM901046 |
CD43+/41+/235+ progenitors generated from T21/wtGATA1 iPSC line, biological rep6 |
| GSM901047 |
CD43+/41+/235+ progenitors generated from T21/GATA1s iPSC line, biological rep1 |
| GSM901048 |
CD43+/41+/235+ progenitors generated from T21/GATA1s iPSC line, biological rep2 |
| GSM901049 |
CD43+/41+/235+ progenitors generated from T21/GATA1s iPSC line, biological rep3 |
| GSM1517424 |
CD43+/41+/235+ progenitors generated from euploid/wtGATA1 iPSC line, biological rep1 |
| GSM1517425 |
CD43+/41+/235+ progenitors generated from euploid/wtGATA1 iPSC line, biological rep2 |
| GSM1517426 |
CD43+/41+/235+ progenitors generated from euploid/GATA1s iPSC line, biological rep1 |
| GSM1517427 |
CD43+/41+/235+ progenitors generated from euploid/GATA1s iPSC line, biological rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE61933 |
Pluripotent stem cells reveal novel erythroid activities of the GATA1 N-terminus |
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| Relations |
| BioProject |
PRJNA156717 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36787_RAW.tar |
48.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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