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Series GSE36804 Query DataSets for GSE36804
Status Public on Jun 21, 2012
Title Expression analysis of genes regulated by RBPJ (Notch) and Axin2 (Wnt) mediated signaling in mouse cardiogenesis at E9.25
Organism Mus musculus
Experiment type Expression profiling by array
Summary Progenitor cells of the first and second heart fields (FHF and SHF) depend on cardiac-specific transcription factors for their differentiation. In mouse mutant embryos, we define the hierarchy of signaling events that controls the expression of cardiac-specific transcription factors during commitment of SHF progenitors at E9.25. Wnt and Bmp act downstream of Notch/RBPJ at this developmental stage. Mutation of Axin2, the negative regulator of canonical Wnt signaling, enhances Wnt and Bmp signals and suffices to rescue the cardiac differentiation arrest caused by loss of RBPJ. By analysis of isolated cardiac progenitors, embryo cultures in the presence of pharmacological inhibitors, and Bmp triple mutants, we could classify the expression of heart-specific transcription factors of SHF progenitors according to their dependence on either Wnt or Bmp signals, Nkx2-5, Isl1, Baf60c and Gata4, SRF, Mef2c, respectively.
 
Overall design Total RNA from whole embryonic hearts of control mice was compared to MesP1-cre:RBPJlox/lox (KO), MesP1-cre:RBPJlox/lox//Axin2-/- (DKO), MesP1-cre:RBPJlox/+//Axin2-/- (hetDKO) and MesP1-cre:RBPJlox/lox//Axin2+/- (DKOhet) mutant mouse embryos.
 
Contributor(s) Klaus A, Birchmeier W
Citation(s) 22711842
Submission date Mar 26, 2012
Last update date Jun 14, 2018
Contact name Alexandra Klaus
E-mail(s) aklaus@mdc-berlin.de
Phone +49 30 9406 3791
Fax +49 30 9406 2656
Organization name MDC (Max Delbrück Center)
Department Signal Transduction, Invasion and Metastasis of Epithelial Cells
Lab Prof.Walter Birchmeier
Street address Robert-Roessle-Str.10
City Berlin
State/province Berlin
ZIP/Postal code 13125
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM901264 control1
GSM901265 control3
GSM901266 control2
Relations
BioProject PRJNA156829

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36804_RAW.tar 3.1 Mb (http)(custom) TAR
GSE36804_non_normalized.txt.gz 1.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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