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| Status |
Public on Mar 29, 2012 |
| Title |
Gene expression analysis in Panc-1 cells in response to treatment with Gli3T, a dominant-negative repressor of Gli |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most aggressive human malignancies. In our studies, we find that the Gli transcription factors are required for Kras initiation of pancreatic tumorigenesis. In order to identify the downstream transcriptional targets of Gli in PDAC, we conducted gene expression analysis using Gli3T, a transcriptional repressor of Gli.
In this data set we include the expression data from 6 samples with three of them expressing a control vector and another three expressing Gli3T
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| Overall design |
Gli3T with IRES-GFP, or an empty vector was ectopically expressed in Panc-1 cells using lipofectamine transfection. GFP positive cells were sorted 24 hours post transfection and RNA was isolated from the cells using Qiagen RNAeasy kit. Affymetrix gene expression array was conducted on the Panc-1 cells expressing the control vector and cells expressing Gli3T. Relative gene expression between the two sample sets was analyzed. Three samples were analyzed for each set.
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| Contributor(s) |
Rajurkar M, Mao J |
| Citation(s) |
22493246 |
| Submission date |
Mar 27, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Mihir Rajurkar |
| E-mail(s) |
mihir.rajurkar@umassmed.edu
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| Phone |
5088563995
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| Organization name |
UMASS Medical School
|
| Department |
Cancer Biology
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| Lab |
Junhao Mao
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| Street address |
364 Plantation st, 470Q LRB
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| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01604 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA157135 |
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