|
| Status |
Public on Mar 29, 2012 |
| Title |
Cell elasticity determines macrophage function. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We report that macrophage elasticity plays a dominant role in bacterial phagocytosis, release of TNF-alpha, and production of reactive oxygen species. We show that macrophage elasticity is modulated by mechanical factors including substrate rigidity and substrate stretch. Changes in macrophage elasticity are dependent upon the degree of actin polymerization, and mediated in part through small rhoGTPase activity. Moreover, the functional effects of macrophage elasticity are not predicted by gene expression profiles.
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| |
|
| Overall design |
Murine RAW 267.4 macrophages were separately grown on 2 matrix stiffness levels (1200, 150000 Pascals) for 0, 2, 6, 18 hours with 3 replicate sample experiments per condition. Total RNA extracted from the cells and profiled by microarrays.
Keywords: Murine RAW 267.4 macrophage, matrix stiffness, phagocytosis, cell elasticity.
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| |
|
| Contributor(s) |
Kho AT, Patel NR, Koziel H |
| Citation(s) |
23028423 |
| Submission date |
Mar 28, 2012 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Alvin T. Kho |
| E-mail(s) |
alvin_kho@hms.harvard.edu
|
| Phone |
617-919-2182
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (21)
|
| GSM904732 |
Macrophage_Control_0 hrs_replicate a |
| GSM904733 |
Macrophage_Control_0 hrs_replicate b |
| GSM904734 |
Macrophage_Control_0 hrs_replicate c |
| GSM904735 |
Macrophage_1.2k Pa_2 hrs_replicate a |
| GSM904736 |
Macrophage_1.2k Pa_2 hrs_replicate b |
| GSM904737 |
Macrophage_1.2k Pa_2 hrs_replicate c |
| GSM904738 |
Macrophage_150k Pa_2 hrs_replicate a |
| GSM904739 |
Macrophage_150k Pa_2 hrs_replicate b |
| GSM904740 |
Macrophage_150k Pa_2 hrs_replicate c |
| GSM904741 |
Macrophage_1.2k Pa_6 hrs_replicate a |
| GSM904742 |
Macrophage_1.2k Pa_6 hrs_replicate b |
| GSM904743 |
Macrophage_1.2k Pa_6 hrs_replicate c |
| GSM904744 |
Macrophage_150k Pa_6 hrs_replicate a |
| GSM904745 |
Macrophage_150k Pa_6 hrs_replicate b |
| GSM904746 |
Macrophage_150k Pa_6 hrs_replicate c |
| GSM904747 |
Macrophage_1.2k Pa_18 hrs_replicate a |
| GSM904748 |
Macrophage_1.2k Pa_18 hrs_replicate b |
| GSM904749 |
Macrophage_1.2k Pa_18 hrs_replicate c |
| GSM904750 |
Macrophage_150k Pa_18 hrs_replicate a |
| GSM904751 |
Macrophage_150k Pa_18 hrs_replicate b |
| GSM904752 |
Macrophage_150k Pa_18 hrs_replicate c |
|
| Relations |
| BioProject |
PRJNA157143 |
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