|
| Status |
Public on Nov 01, 2012 |
| Title |
Spreading of X-chromosome inactivation via a hierarchy of defined Polycomb stations |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Binding of Polycomb repressive complex 2 (PRC2) and chromatin composition of the inactive X (Xi) before, during and after X chromosome inactivation reveal that spreading is driven by a combination of Xi-specific strong and moderate Ezh2 sites. Sequence context of these sites shows a moderate enrichment of SINEs and simple repeats. The general pattern of Ezh2 and H3K27me3 distribution over the chromosome reflect a graded concentration originating from strong Ezh2 sites, around which moderate sites are clustered, suggesting a hierarchy of Ezh2 sites govern spreading.
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| |
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| Overall design |
ChIP-seq of Ezh2 and H3K27me3 as well as the three active marks H3K4me3, H3K36me3 and RNA-POLII-serine5 phosphorylation (RNA-polII-S5P) in female cell lines: undifferentiated embryonic stem (ES) cells (d0), differentiating ES cells (d7) and a transformed embryonic fibroblast cell line (MEF).
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| |
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| Contributor(s) |
Pinter SF, Sadreyev RI, Yildirim E, Jeon Y, Ohsumi TK, Borowsky M, Lee JT |
| Citation(s) |
22948768, 23564346 |
| Submission date |
Mar 28, 2012 |
| Last update date |
Jul 22, 2022 |
| Contact name |
Ruslan Sadreyev |
| E-mail(s) |
rsadreyev@mgh.harvard.edu
|
| Phone |
6176435697
|
| Organization name |
Harvard Medical School/ Mass General Hospital
|
| Street address |
185 Cambridge St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (20)
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|
| Relations |
| BioProject |
PRJNA157029 |
| SRA |
SRP028295 |
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