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| Status |
Public on Jul 04, 2012 |
| Title |
Site-specific cytosine-5 methylation by the mouse RNA methyltransferases Dnmt2 and NSun2 promotes tRNA stability and protein synthesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse embryonic fibroblast (MEFs) cell lines and liver samples from Dnmt2 and Nsun2 single mutant, double mutant and wildtype mice were used to identify potential mRNA substrates of both proteins.
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| Overall design |
MEFs were derived from day 13.5 embryos and immortalized by transfection with a plasmid expressing the SV40 large-T antigen. Total RNA of MEFs cell line at passage 9 and total RNA extracted from 2 months old mouse liver tissues of Dnmt2, Nsun2 single mutant, double mutant and wildtype mice were arrayed on Illumina MouseRef8 v2 chips.
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| Contributor(s) |
Tuorto F, Liebers R, Musch T, Schaefer M, Hofmann S, Kellner S, Frye M, Helm M, Stöcklin G, Lyko F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 29, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Francesca Tuorto |
| E-mail(s) |
f.tuorto@dkfz.de
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| Organization name |
German Cancer Research Center
|
| Department |
Epigenetics
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| Street address |
Im Neuenheimer Feld 580
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| City |
Heidelberg |
| ZIP/Postal code |
D-69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA157093 |
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