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Series GSE36918 Query DataSets for GSE36918
Status Public on Jul 04, 2012
Title Site-specific cytosine-5 methylation by the mouse RNA methyltransferases Dnmt2 and NSun2 promotes tRNA stability and protein synthesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse embryonic fibroblast (MEFs) cell lines and liver samples from Dnmt2 and Nsun2 single mutant, double mutant and wildtype mice were used to identify potential mRNA substrates of both proteins.
 
Overall design MEFs were derived from day 13.5 embryos and immortalized by transfection with a plasmid expressing the SV40 large-T antigen. Total RNA of MEFs cell line at passage 9 and total RNA extracted from 2 months old mouse liver tissues of Dnmt2, Nsun2 single mutant, double mutant and wildtype mice were arrayed on Illumina MouseRef8 v2 chips.
 
Contributor(s) Tuorto F, Liebers R, Musch T, Schaefer M, Hofmann S, Kellner S, Frye M, Helm M, Stöcklin G, Lyko F
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Submission date Mar 29, 2012
Last update date Jun 14, 2018
Contact name Francesca Tuorto
E-mail(s) f.tuorto@dkfz.de
Organization name German Cancer Research Center
Department Epigenetics
Street address Im Neuenheimer Feld 580
City Heidelberg
ZIP/Postal code D-69120
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM906315 MEFs wt
GSM906316 MEFs Dnmt2
GSM906317 MEFs Nsun2
Relations
BioProject PRJNA157093

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36918_RAW.tar 3.1 Mb (http)(custom) TAR
GSE36918_non_normalized.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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