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| Status |
Public on Aug 25, 2012 |
| Title |
Elevating Sox2 levels deleteriously affects the growth of glioblastoma and medulloblastoma cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Induction of the transcription factor Sox2 from a doxycycline-inducible promoter in iSox2-DAOY medulloblastoma cells.
Affymetrix microarrays were used to characterize the gene expression profile of iSox2-DAOY cells in the absence and presence of doxycycline.
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| Overall design |
Human DAOY medulloblastoma cells were engineered to express epitope tagged Sox2 under the control of a doxycycline inducible promoter, to produce iSox2-DAOY cells. iSox2-DAOY cells were FACS sorted into CD133 low and high populations. The CD133 low population was cultured in the absence and presence of 0.5 µg/mL doxycycline for 24 hours. RNA was extracted from cells cultured without and with doxycycline, and was used for microarray analysis.
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| Contributor(s) |
Cox JL, Wilder PJ, Rizzino A |
| Citation(s) |
22937156 |
| Submission date |
Mar 29, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Angie Rizzinio |
| E-mail(s) |
arizzino@unmc.edu
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| Organization name |
University of Nebraska Medical Center
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| Department |
Eppley Institute for Research in Cancer and Allied Diseases
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| Street address |
986805 Nebraska Medical Center
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| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198-6805 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA157109 |
