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| Status |
Public on Mar 31, 2012 |
| Title |
Genome-wide analysis of transcription factor FoxO1-responsive gene expression in the ventromedial hypothalamus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of gene expression regulated by FoxO1 in the ventromedial hypothalamus (VMH) of wildtype and knockout mice. Results provide important information of gene expression in the VMH.
The transcription factor FoxO1 contributes to leptin and insulin action, including cells in the brain. However, the neurons mediating these effects and the identity of the molecular targets through which FoxO1 exerts metabolic actions remains to be defined.
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| Overall design |
For the gene expression profiling, total RNA was obtained from the VMH of WT and VMH-specific FoxO1 KO mice using Qiagen RNeasy Lipid Tissue Mini Kit (Qiagen Sciences, Maryland) and Phase Lock Gel (5 Prime Inc., Gaithersburg, MD). To make sure reproducibility and biological significance, triple hybridizations were performed for each genotype with the RNA from three independent VMH samples, each sample contains RNA from three animals (biological replicates). Genomics and Microarray Core Facility at UT-Southwestern (http://microarray.swmed.edu/) checked RNA quality with Bioanalyzer Chip and processed the samples for hybridization with Illumina Mouse-6 V2 BeadChip (Illumina Inc., San Diego, CA). We used Partek Genomics Suite 6.5 (Partek Inc., St Louis, MO) and Ingenuity Pathway Analysis (Ingenuity Systems, Inc., Redwood City, CA) for data analysis.
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| Contributor(s) |
Kim KW, Elmquist JK |
| Citation missing |
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| Submission date |
Mar 29, 2012 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Ki Woo Kim |
| E-mail(s) |
kiwoo.kim@utsouthwestern.edu
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| Organization name |
UT-Southwestern
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| Department |
Internal Medicine and Pharmacology
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| Street address |
5323 Harry Hines Blvd.
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| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA157341 |
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