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| Status |
Public on Sep 24, 2012 |
| Title |
Long-term culture associated gene expression changes in MSC [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Mesenchymal stromal cells (MSC) were isolated from human bone marrow. Here, we have compared gene expression profiles of MSC at early and late passages. Long-term culture associated gene expression changes were then correlated with DNA-methylation profiles. The goal of this study was to determine if senescence-associated DNA-methylation (SA-DNAm) changes are reflected by differential gene expression. Overall, genes with SA-DNAm changes (particularly with SA-hypomethylation) were detected at low level and seemed to be scarcely expressed at early and late passages.
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| Overall design |
MSC were isolated from three different donors and culture expanded until replicative senescence. Gene expression profiles were compared at early and late passage using GeneChip Humang Gene 1.0 ST arrays (Affymetrix). Six hybridizations are included in this series.
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| |
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| Contributor(s) |
Koch CM, Lin Q, Walenda G, Wagner W |
| Citation(s) |
23032973, 23080539 |
| Submission date |
Apr 05, 2012 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
wwagner@ukaachen.de
|
| Phone |
+49 241 8088611
|
| Organization name |
RWTH Aachen University
|
| Department |
Helmholtz Institute for Biomedical Engineering
|
| Lab |
Stem Cell Biology and Cellular Engineering
|
| Street address |
Pauwelsstrasse 20
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE37067 |
Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes |
|
| Relations |
| BioProject |
PRJNA158005 |
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