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| Status |
Public on Sep 24, 2012 |
| Title |
Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes [Illumina] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Pluripotent stem cells evade replicative senescence, whereas other primary cells lose their proliferation and differentiation potential after a limited number of cell divisions – and this is accompanied by specific senescence-associated DNA methylation (SA-DNAm) changes. Here, we investigate SA-DNAm changes in mesenchymal stromal cells (MSC) upon long-term culture, irradiation-induced senescence, immortalization and reprogramming into induced pluripotent stem cells (iPSC) using high density HumanMethylation450 BeadChips. SA-DNAm changes are highly reproducible and occur particularly in intergenic and non-promoter regions of developmental genes. We demonstrate that ionizing irradiation, although associated with a very similar senescence phenotype, does not affect SA-DNAm. Furthermore, overexpression of the catalytic subunit of the human telomerase (TERT) or conditional immortalization with a doxycycline-inducible system (TERT and SV40 TAg) result in telomere extension but do not influence SA-DNAm. In contrast, we demonstrate that reprogramming into iPSC prevented SA-DNAm changes. Our results indicate that replicative senescence is associated with an epigenetically controlled process which stalls cells in a particular differentiated state, whereas irradiation-induced senescence and immortalization are not causally related to this process. Absence of SA-DNAm in pluripotent cells may play a central role for their escape from cellular senescence.
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| Overall design |
Samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
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| Contributor(s) |
Koch CM, Reck K, Shao K, Lin Q, Ziegler P, Joussen S, Walenda G, Drescher W, Opalka B, May T, Brümmendorf T, Zenke M, Saric T, Wagner W |
| Citation(s) |
23032973, 23080539 |
| Submission date |
Apr 05, 2012 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
wwagner@ukaachen.de
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| Phone |
+49 241 8088611
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| Organization name |
RWTH Aachen University
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| Department |
Helmholtz Institute for Biomedical Engineering
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| Lab |
Stem Cell Biology and Cellular Engineering
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| Street address |
Pauwelsstrasse 20
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| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE37067 |
Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes |
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| Relations |
| BioProject |
PRJNA158007 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37066_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE37066_raw_AB_intensities.txt.gz |
42.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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