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| Status |
Public on Apr 07, 2015 |
| Title |
Gene expression signatures of MEF2D-silencing hepatocellular carcinoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Global expression profiles in Huh7 after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, were compared to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle.
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| Overall design |
In order to investigate the molecular mechanisms involved in MEF2D-mediated regulation of cell cycle, we assessed gene expression profiles in Huh7 cells after infection with Lv-shMEF2D and Lv-scrambled, as well as transfection with siRNA-MEF2D and siRNA-control, by cDNA microarray. Analysis of global mRNA expression profile indicated a shift toward G2/M arrest in the cells after downregulating MEF2D expression. The genes that inhibit G2/M transition were found to be expressed in high level in MEF2D-downregulated group, as compared with control group. Meanwhile, mRNA abundance of G2/M transition-promoting genes, except CDC2 and CDC25C, was reduced when MEF2D expression was depressed in Huh7 cells
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| Contributor(s) |
Ma L, Liu J, Liu L, Duan G, Wang Q, Xu Y, Xia F, Shan J, Shen J, Yang Z, Yan X, Bie P, Cui Y, Bian X, Qian C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 09, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
leina ma |
| Organization name |
Third Military Medical University
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| Department |
Southwest Hospital
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| Lab |
Institute of Pathology and Southwest Cancer Center
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| Street address |
Gaotanyan Main Street, No. 35
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| City |
ChongQing |
| ZIP/Postal code |
400038 |
| Country |
China |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA158281 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37123_RAW.tar |
8.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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