|
| Status |
Public on Nov 01, 2012 |
| Title |
Gene expression data from time course of fin regeneration in Danio rerio (part 1) |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Olsen et al (2010) have shown that induced Diabetes mellitus (DM) in adult Zebrafish results in an impairment of tissue regeneration as monitored by caudal fin regeneration. In those studies, streptozocin was used to induce hyperglycemia in adult zebrafish, and then, following streptozocin withdrawal, a recovery phase was allowed to re-establish euglycemia, due to pancreatic b-cell regeneration. DM-associated impaired fin regeneration continued indefinitely in the metabolic memory state (MM); allowing for subsequent molecular analysis of the underlying mechanisms of MM. This study focuses on elucidating the molecular basis explaining DM-associated impaired fin regeneration and why it persists into the MM state. The analysis of microarray data indicated that of the 14,900 transcripts analyzed, aberrant expression of 71 genes relating to tissue developmental and regeneration processes were identified in DM fish and the aberrant expression of these 71 genes persisted into the MM state. Key regulatory genes of major signal transduction pathways were identified among this group of 71; and therefore, these findings provide a possible explanation for how hyperglycemia induces impaired fin regeneration and why it continues into the MM state.
|
| |
|
| Overall design |
Total RNA was extracted from caudal fin at 0, 12, 24 and 48 hours post amputation from untreated controls and metabolic memory zebrafish.
|
| |
|
| Contributor(s) |
Sarras MP, Leontovich AA, Olsen AS, Pisano G, Intine RV |
| Citation(s) |
23438205 |
| Submission date |
Apr 11, 2012 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Alexey Aleksandrovich Leontovich |
| E-mail(s) |
leontovich.alexey@mayo.edu
|
| Phone |
507-284-4850
|
| Organization name |
Mayo Clinic
|
| Department |
Health Science Research
|
| Lab |
Biomedical Statistics and Informatics
|
| Street address |
200 First street
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55902 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE37165 |
Gene expression data from time course of fin regeneration in Danio rerio |
|
| Relations |
| BioProject |
PRJNA158641 |
Less...
More...