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Series GSE37163 Query DataSets for GSE37163
Status Public on Nov 01, 2012
Title Gene expression data from time course of fin regeneration in Danio rerio (part 1)
Organism Danio rerio
Experiment type Expression profiling by array
Summary Olsen et al (2010) have shown that induced Diabetes mellitus (DM) in adult Zebrafish results in an impairment of tissue regeneration as monitored by caudal fin regeneration. In those studies, streptozocin was used to induce hyperglycemia in adult zebrafish, and then, following streptozocin withdrawal, a recovery phase was allowed to re-establish euglycemia, due to pancreatic b-cell regeneration. DM-associated impaired fin regeneration continued indefinitely in the metabolic memory state (MM); allowing for subsequent molecular analysis of the underlying mechanisms of MM. This study focuses on elucidating the molecular basis explaining DM-associated impaired fin regeneration and why it persists into the MM state. The analysis of microarray data indicated that of the 14,900 transcripts analyzed, aberrant expression of 71 genes relating to tissue developmental and regeneration processes were identified in DM fish and the aberrant expression of these 71 genes persisted into the MM state. Key regulatory genes of major signal transduction pathways were identified among this group of 71; and therefore, these findings provide a possible explanation for how hyperglycemia induces impaired fin regeneration and why it continues into the MM state.
Overall design Total RNA was extracted from caudal fin at 0, 12, 24 and 48 hours post amputation from untreated controls and metabolic memory zebrafish.
Contributor(s) Sarras MP, Leontovich AA, Olsen AS, Pisano G, Intine RV
Citation(s) 23438205
Submission date Apr 11, 2012
Last update date Jan 25, 2018
Contact name Alexey Aleksandrovich Leontovich
Phone 507-284-4850
Organization name Mayo Clinic
Department Health Science Research
Lab Biomedical Statistics and Informatics
Street address 200 First street
City Rochester
State/province MN
ZIP/Postal code 55902
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (24)
GSM912663 Control (MM) 0h rep 1
GSM912664 Control (MM) 0h rep 2
GSM912665 Control (MM) 0h rep 3
This SubSeries is part of SuperSeries:
GSE37165 Gene expression data from time course of fin regeneration in Danio rerio
BioProject PRJNA158641

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37163_RAW.tar 49.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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