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| Status |
Public on Jun 16, 2012 |
| Title |
Genome-wide analysis of three clonal neural stem cell lines derived from human foetal spinal cord tissue. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Stem cells have received substantial interest both for their potential as in vitro tools to study development and as potential therapeutic agents in a range of degenerative diseases of the nervous system. We have generated clonal neural stem cell lines from human foetal spinal cord conditionally immortalised with 4-hydroxy tamoxifen inducible cMyc (cMycERTAM), and performed a detailed assessment of their identity in terms of their expression of homeodomain transcription factors and their capacity to generate particular neuronal subtypes of the spinal cord. These lines retain a ventral spinal cord progenitor phenotype and give rise to electrically active neuronal subtypes characteristic of specific ventral progenitor subdomains. Upon grafting into lesioned rat spinal cord these cells differentiate into ChAT+ve motorneurons and show robust survival after 4 months.
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| Overall design |
Total RNA was obtained from three proliferative undifferentiated neural stem cell lines at 75% confluence in culture. Two replicates of each clonal line were generated for microarray analysis.
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| Contributor(s) |
Cocks G, Jeffries A, Price J |
| Citation missing |
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| Submission date |
Apr 16, 2012 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Graham Cocks |
| Organization name |
King's College London
|
| Department |
Neuroscience
|
| Lab |
Jack Price
|
| Street address |
The James Black Centre, 125 Coldharbour Lane
|
| City |
London |
| ZIP/Postal code |
SE5 9NU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (6)
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| GSM915445 |
SPC-01 Undifferentiated Replicate 1 |
| GSM915446 |
SPC-01 Undifferentiated Replicate 2 |
| GSM915447 |
SPC-04 Undifferentiated Replicate 1 |
| GSM915448 |
SPC-04 Undifferentiated Replicate 2 |
| GSM915449 |
SPC-06 Undifferentiated Replicate 1 |
| GSM915450 |
SPC-06 Undifferentiated Replicate 2 |
|
| Relations |
| BioProject |
PRJNA159397 |
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