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Series GSE37312 Query DataSets for GSE37312
Status Public on Mar 01, 2013
Title Gene expression profiling of rhesus macaques vaccinated with ALVAC-SIVgpe DNA + SIVgp120 protein subunit and unvaccinated controls after challenge with SIVmac251 - 11 wks post-infection
Organism Macaca mulatta
Experiment type Expression profiling by array
Summary The SIVmac251 macaque model has been used to evaluate the efficacy of vaccine for HIV. Exposure of macaques to a single high dose of SIVmac251 results in transmission of multiple viral variants, which contrasts the few HIV variants typically transmitted in humans. In here, we investigated whether the dose of SIVmac251 challenge affected vaccination efficacy and found that exposure of the immunized macaques to single high dose of SIVmac251 resulted in no vaccine efficacy, whereas exposure to a tenfold lower dose resulted in protection from SIVmac251 acquisition and protection from disease in animals that become infected. The dose of challenge did not affect the expression of inflammatory genes in the gut in acute infection, but at set point, a significant down regulation of interferon responsive genes and up regulation of genes involved in B and T-cell responses, was observed only in vaccinated animals exposed to a lower dose of SIVmac251. Accordingly, in these animals, we also found a significant correlation with vaccine induced T-cell responses and protection from disease. These data demonstrate that the evaluation of the efficacy of vaccine candidates for HIV relies on accurate modeling in macaques to better mimic HIV transmission to humans.
 
Overall design A total of 34 RNA samples were hybridized on to Rhesus Affymetrix 3' Expression arrays. The study was composed of 9 vaccinated and 9 control animals subjected to a low dose challenge and 7 vaccinated and 9 control animals subjected to a high dose challenge
 
Contributor(s) Vaccari M, Keele BF, Bosinger SE, Doster M, Ma Z, Pollara J, Hryniewicz A, Ferrari G, Guan Y, Forthal D, Venzon D, Fenizia C, Morgan T, Montefiori D, Lifson JD, Miller C, Silvestri G, Felber B, Pavlakis G, Tartaglia J, Franchini G
Citation(s) 23325681
Submission date Apr 16, 2012
Last update date Apr 04, 2013
Contact name Gregory K Tharp
E-mail(s) gktharp@emory.edu
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
 
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (34)
GSM915855 Rectal mucosal biopsy_ALVAC+_low dose challenge_Animal P008
GSM915856 Rectal mucosal biopsy_ALVAC+_low dose challenge_Animal M898
GSM915857 Rectal mucosal biopsy_ALVAC+_low dose challenge_Animal P009
This SubSeries is part of SuperSeries:
GSE37320 Gene expression profiling of rhesus macaques vaccinated with ALVAC-SIVgpe DNA + SIVgp120 protein subunit and unvaccinated controls after challenge with SIVmac251
Relations
BioProject PRJNA159477

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37312_RAW.tar 143.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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