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Series GSE37323 Query DataSets for GSE37323
Status Public on May 01, 2013
Title Genome-wide reprogramming of the chromatin landscape underlies endocrine therapy resistance in breast cancer
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The estrogen receptor alpha (ERa) drives the growth of two-thirds of all breast cancers. Endocrine therapy impinges on estrogen-induced ERa activation to block tumor growth. However, half of ERa-positive breast cancers are tolerant or acquire endocrine therapy resistance. Here we demonstrate that breast cancer cells undergo genome-wide reprogramming of their chromatin landscape, defined by epigenomic maps and chromatin openness, as they acquire resistance to endocrine therapy. This reveals a role for the Notch pathway while excluding classical ERa signaling. In agreement, blocking Notch signaling, using gamma-secretase inhibitors, or targeting its downstream gene PBX1 abrogates growth of endocrine therapy-resistant breast cancer cells. Moreover Notch signaling through PBX1 directs a transcriptional program predictive of tumor outcome and endocrine therapy response.
 
Overall design Comparing histone modifications (H3K4me2 and H3K36me3), chromatin openness (FAIRE) and PBX1 binding between endocrine therapy sensitive MCF7 and resistant MCF7-LTED cells.
 
Contributor(s) Zhang X
Citation(s) 23576735
Submission date Apr 16, 2012
Last update date May 15, 2019
Contact name xiaoyang zhang
E-mail(s) xiaoyang.zhang@dartmouth.edu
Organization name Dartmouth Medical School
Street address 1 Medical Center Driver
City Lebanon
ZIP/Postal code 03756
Country USA
 
Platforms (2)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (9)
GSM916106 H3K36me3_untreated
GSM916107 H3K36me3_E2 treated
GSM916108 H3K4me2_LTED
This SubSeries is part of SuperSeries:
GSE39418 Genome-wide reprogramming of the chromatin landscape underlies endocrine therapy resistance in breast cancer
Relations
BioProject PRJNA159579
SRA SRP012270

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37323_RAW.tar 4.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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