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| Status |
Public on Apr 18, 2012 |
| Title |
HepG2 treated with Polycyclic Aromatic Hydrocarbons (PAHs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Assessing the potential carcinogenicity of human toxins represents an ongoing challenge. Chronic rodent bioassays predict human cancer risk with limited reliability, and are expensive and time-consuming. To identify alternative prediction methods, we evaluated a transcriptomics-based human in vitro model to classify carcinogens by their modes of action. The aim of this study was to determine the transcriptomic response and identify specific molecular signatures of polycyclic aromatic hydrocarbons (PAHs), which can be used as predictors of carcinogenicity of environmental toxins in human in vitro systems. We found that characteristic molecular signatures facilitate identification and prediction of carcinogens.
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| Overall design |
To evaluate the change in gene expression levels, human hepatocellular carcinoma (HepG2) cells were exposed to nine different PAHs (benzo[a]pyrene, dibenzo[a,h]anthracene, 3-methylcholanthrene, naphthalene, chrysene, phenanthrene, benzo[a]anthracene, benzo[k]fluoranthene, and indeno[1,2,3-c,d]pyrene) for 48 h. Gene expression analysis was conducted using a 44K whole human genome microarray (Agilent Technologies, USA).
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| Contributor(s) |
Ryu J, Song M, Song M, Choi H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 17, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Mi-Kyung Song |
| E-mail(s) |
lovely86c@nate.com
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| Phone |
82-2-958-6799
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| Organization name |
Korea Institute of Science & Technology
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| Street address |
39-1 Hwawol-gok-dong, Wolsong-gil5, Seongbuk-gu-seoul
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| City |
Seoul |
| ZIP/Postal code |
136-791 |
| Country |
South Korea |
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| Platforms (2) |
| GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA159765 |
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