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Series GSE37344 Query DataSets for GSE37344
Status Public on Sep 23, 2014
Title PGC-1α mediates mitochondrial biogenesis and oxidative phosphorylation in cancer cells to promote metastasis
Organism Mus musculus
Experiment type Expression profiling by array
Summary The study aimed to analyse the transcriptome of mouse cancer cells while in primary tumor, in circulation and after homing to metastatic site. The model used here is the 4T1 cancer cell orthotopic model.
Overall design GFP-labeled 4T1 breast cancer cells were orthotopically implanted in the mammary pads of mice. In this mouse model for breast cancer, primary breast tumors emerge following injection of cancer cells in the breast pad of female mice and subsequently develop lung metastases with 100% penetrance. Circulating cancer cells (CCC) and cancer cells from the primary tumors (PCC) and metastatic lungs (MCC) were FACS purified and their transcriptome assayed by gene expression microarray. RNA was extracted from PCC, MCC, and CCC using RNeasy Plus Mini Kit (Qiagen) and submitted to the Molecular Genetics Core Facility at Children’s Hospital (Boston, MA). Microarray analysis was performed using Mouse Ref8 Gene Expression BeadChip (Illumina platform).
Contributor(s) LeBleu V, Kalluri R
Citation(s) 25241037
Submission date Apr 17, 2012
Last update date Jun 14, 2018
Contact name Valerie LeBleu
Organization name MDACC
Street address 1881 East Road
City Houston
State/province TX
ZIP/Postal code 77054
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (3)
GSM916516 PCC
GSM916517 CCC
GSM916518 MCC
BioProject PRJNA159603

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37344_RAW.tar 3.1 Mb (http)(custom) TAR
GSE37344_non-normalized.txt.gz 626.5 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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