|
| Status |
Public on Apr 25, 2016 |
| Title |
Genomic and functional studies in T-Acute Lymphoblastic Leukemia patients exhibiting clonal T Cell Receptor γ and δ gene rearrangement |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
T-cell Acute Lymphoblastic Leukemia (T-ALL) is a lymphoid malignancy characterized by clonal proliferation of immature thymocytes arrested at particular stage of differentiation. Earlier studies from our lab have demonstrated that 30% of Indian T-ALL patients exhibited clonal rearrangement of both TCR γ and δ genes. The survival was higher in T-ALL patients showing clonal rearrangements for TCR γ and δ genes. In the present study, we aimed at investigating the gene expression profiles of TCR γδ+ T-ALL patients and correlating it with functional analysis to understand the reasons for increased survival in these patients. Our studies demonstrate differential expression of genes in TCR γδ clonal T-ALL compared to non TCR γδ clonal T-ALL patients. Functional annotation of significantly upregulated genes (p<0.05) in TCR γδ+ T-ALL patients showed involvement in antigen processing and presentation, cell-cell communication, apoptosis, cell adhesion molecules and immune synapse pathways. Expression of eight selected genes (SMAD3, NFkB1, HLA-DQB1, SOCS2, CISH, FASLG, RXRA,and IFNβ1) was validated in TCRαβ+ and TCRγδ+ subgroups of T-ALL patients using quantitative Real-time PCR. Studies focusing on functional aspects of the gene(s) identified from gene expression profile would aid in developing future therapeutic strategies.
|
| |
|
| Overall design |
Agilent one-color experiment,Organism: Human ,Agilent-014850 Whole Human Genome Microarray 4x44K G4112F , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
|
| |
|
| Contributor(s) |
Bhat J, Mirji G, Banavali S, Chiplunkar S |
| Citation(s) |
27070758 |
| Submission date |
Apr 18, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Shubhada Chiplunkar |
| E-mail(s) |
schiplunkar@actrec.gov.in
|
| Phone |
022-27405032
|
| Organization name |
Advanced Centre For Treatment Research And Education in Cancer
|
| Department |
Immunology
|
| Lab |
Chiplunkar
|
| Street address |
Sector no.22
|
| City |
Kharghar |
| State/province |
Maharashtra |
| ZIP/Postal code |
410210 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA159717 |
Less...
More...