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Series GSE37485 Query DataSets for GSE37485
Status Public on May 03, 2012
Title Expression data of pre-stasis cultured HMEC
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have generated a large collection of normal human mammary epithelial cell strains from women aged 16 to 91 years, derived from primary tissues, to enable functional and molecular interrogation of aging. We demonstrate in finite-lifespan cultured and uncultured epithelial cells that aging is associated with reduction of myoepithelial cells and with increases in luminal cells expressing keratin 14 and integrin α6, traits that are expressed exclusively in myoepithelial cells in women under 30. We find that changes to the luminal lineage result from age-dependent expansion of multipotent progenitors that bear defects resulting in incompletely differentiated luminal cells. These findings were verified in vivo in normal breast tissues. Myoepithelial cells have been suggested to act as tumor suppressors, and progenitor cells are implicated as the etiological roots of mammary carcinomas. Thus with aging there is a shift in the balance of luminal/myoepithelial lineages, and changes in the functional spectrum of multipotent progenitors, which presages increased potential for malignant transformation.
 
Overall design Finite lifespan pre-stasis HMEC from specimens from reduction mammoplasties of 7 patients of different age were collected. The expression data was queried for different biological replicates and at different passages. For strain 240, luminal and myoepithelial cells were collected using flow cytometry.
 
Contributor(s) LaBarge MA, Garbe JC, Pepin F, Stampfer MR
Citation(s) 22552289
Submission date Apr 23, 2012
Last update date Jan 17, 2017
Contact name Francois Pepin
Organization name McGill University
Street address 1160 Pine Av W
City Montreal
State/province Quebec
ZIP/Postal code H3A1A3
Country Canada
 
Platforms (1)
GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
Samples (52)
GSM921274 X122L.p2AM87ACTX_Pre-stasis HMEC Biological replicate #1
GSM921275 X122L.p2BM87ACTX_Pre-stasis HMEC Biological replicate #2
GSM921276 X122L.p4M87ACTX_Pre-stasis HMEC
Relations
BioProject PRJNA161509

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37485_RAW.tar 129.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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