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Series GSE37515 Query DataSets for GSE37515
Status Public on Apr 23, 2012
Title Gene expression signatures for fractions of E7.5 whole embryos based on Endoglin and Flk-1 markers
Organism Mus musculus
Experiment type Expression profiling by array
Summary Much remains unknown about the signals that induce early mesoderm to initiate hematopoietic differentiation. Here we show that endoglin (Eng), a receptor for the TGFβ superfamily, identifies all cells with hematopoietic fate in the early embryo. These arise in an Eng+Flk1+ mesodermal precursor population at E7.5, a cell fraction also endowed with endothelial potential. In Eng knockout embryos, hematopoietic colony activity and numbers of CD71+Ter119+ erythroid progenitors were severely reduced. This coincided with severely reduced expression of embryonic globin and key BMP target genes including the hematopoietic regulators Scl, Gata1, Gata2 and Msx-1. To interrogate molecular pathways active in the earliest hematopoietic progenitors, we applied transcriptional profiling to sorted cells from E7.5 embryos. Eng+Flk-1+ progenitors co-expressed TGFβ and BMP receptors and target genes. Furthermore, Eng+Flk-1+ cells presented high levels of phospho-SMAD1/5, indicating active TGFβ and/or BMP signaling. Remarkably, under hematopoietic serum-free culture conditions, hematopoietic outgrowth of endoglin-expressing cells was dependent on TGFβ superfamily ligands: BMP4, BMP2, or TGF-β1. These data demonstrate that the E+F+ fraction at E7.5 represents mesodermal cells competent to respond to TGFb1, BMP4, or BMP2, shaping their hematopoietic development, and that endoglin is a critical regulator in this process by modulating TGF/BMP signaling.
 
Overall design E7.5 pooled embryos (25 litters; 300 embryos approximately) were dissected and 3,000 cells were sorted in triplicate for Eng-Flk1-, Eng-Flk1+, Eng+Flk1+, and Eng+Flk1- fractions. Microarray results were analyzed with GeneSpring GX software.
 
Contributor(s) Borges L, Iacovino M
Citation(s) 22535663
Submission date Apr 23, 2012
Last update date May 10, 2018
Contact name Rita Perlingeiro
E-mail(s) perli032@umn.edu
Organization name University of Minnesota
Department Medicine
Lab Perlingeiro Lab
Street address 312 Church ST SE; NHH 4-124
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (12)
GSM920898 Eng-Flk1-_rep1
GSM920899 Eng-Flk1-_rep2
GSM920900 Eng-Flk1-_rep3
Relations
BioProject PRJNA160973

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37515_K1099_A3550_intensity_norm.txt.gz 4.2 Mb (ftp)(http) TXT
GSE37515_RAW.tar 116.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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