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Series GSE37554 Query DataSets for GSE37554
Status Public on Jul 01, 2012
Title Genome-wide analysis of gene expression in WT and Erk2 deficient CD4 T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Effective immune responses depend upon appropriate T cell differentiation in accord with the nature of an infectious agent, and the contingency of differentiation depends minimally on T cell antigen receptor, co-receptor, and cytokine signals. In this reverse genetic study we show that the Map Kinase, Erk2, is nonessential for T cell proliferation in the presence of optimum co-stimulation. Instead, it has opposite polar effects on T-bet and Gata3 expression and hence on Th1 and Th2 differentiation. Alternatively, in the presence of TGFbeta, the Erk pathway suppresses a large program of gene expression effectively limiting the differentiation of Foxp3+ T reg cells. In the latter case, the mechanisms involved include suppression of Gata3 and Foxp3, induction of Tbx21, phosphorylation of Smad2,3, and possibly suppression of Socs2, a positive inducer of Stat5 signaling. Consequently, loss of Erk2 severely impeded Th1 differentiation while enhancing the development of Foxp3+ induced T regulatory cells. Selected profiles of gene expression under multiple conditions of T cell activation illustrate the opposing consequences of Erk pathway signaling.
 
Overall design Analysis of the Th1 and iTreg differentiation regulated by Erk2.
Total RNA obtained from WT or Erk2 deficient CD4 T cells in different conditions.
 
Contributor(s) Hedrick SM, Chang CF
Citation(s) 22675204
Submission date Apr 24, 2012
Last update date Jun 14, 2018
Contact name Chiungfang Chang
E-mail(s) chc018@ucsd.edu
Phone 858-534-6559
Fax 858-534-0980
Organization name University of California, San Diego
Department Biological Science
Lab NSB 5116
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92122
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM921524 5388876092_A
GSM921525 5388876092_B
GSM921526 5388876092_C
Relations
BioProject PRJNA161521

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37554_RAW.tar 3.1 Mb (http)(custom) TAR
GSE37554_non-normalized_data.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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