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| Status |
Public on May 01, 2013 |
| Title |
The Refilin/Filamin complex regulates filipodia dynamics in oligodendrocyte progenitor cells. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Actin assembly and dynamics control the shape, motility and functions of diverse cell types. Programme for controlling theses dynamics is hard-coded into actin binding proteins and regulatory proteins. Refilins (RefilinA and RefilinB) are short lived regulatory proteins that interact with the actin-binding protein Filamin to convert it from an actin branching protein into one that bundles. We here show that different mechanisms converge to increase Refilin level in brain NG2+ precursor cells (polydendrocytes) committed to differentiate into the oligodendrocyte lineage. RefilinA is up-regulated through transcriptional activation during commitment into oligodendrocyte progenitor cells (OPC). RefilinB expression relies on PDGF signalling and is further stabilized by a unique auto-inhibitory domain masking the adjacent conserved PEST degradation signal. In NG2+ precursor cells and OPC, the RefilinB/Filamin complex localizes on filipodia protrusions and filipodial processes. Stable ectopic expression of Refilins in cells stimulates membrane remodelling dynamics linked with filipodia growth. These studies extend the function of the Refilin/FLNA complex to cell membrane remodelling linked with reorganization of underlying actin cytoskeleton.
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| Overall design |
We performed whole transcriptomic analysis on the three cell population derived from OPC cells. Three different cultures were done and for each experiment we compared each cell population (NG2+, NG2+/A2B5 and O4) to the other 2 cell populations.
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| Contributor(s) |
Gay O, Gilquin B, Wierinckx A, Rey C, Lachuer J, Baudier J, Benaud C, Deshiere A, Assard N, Stuelsatz P, Delphin C, Honnorat J |
| Citation missing |
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| Submission date |
Apr 26, 2012 |
| Last update date |
Jun 06, 2014 |
| Contact name |
jacques Baudier |
| E-mail(s) |
jbaudier@cea.fr
|
| Phone |
33 438 78 43 28
|
| Organization name |
INSERM
|
| Department |
iRTSV
|
| Lab |
U1038
|
| Street address |
17 Rue des Martyrs
|
| City |
Grenoble |
| ZIP/Postal code |
38054 |
| Country |
France |
| |
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| Platforms (1) |
| GPL2896 |
GE Healthcare/Amersham Biosciences CodeLink™ Rat Whole Genome Bioarray |
|
| Samples (9)
|
| GSM923088 |
cells expressing the marker O4 and are NG2 negative: O4-I |
| GSM923089 |
cells expressing the marker O4 and are NG2 negative: O4-II |
| GSM923096 |
cells expressing the marker O4 and are NG2 negative: O4-III |
| GSM923097 |
cells expressing the marker A2B5 and are NG2 positive: NG2/A2B5-I |
| GSM923098 |
cells expressing the marker A2B5 and are NG2 positive: NG2/A2B5-II |
| GSM923099 |
cells expressing the marker A2B5 and are NG2 positive: NG2/A2B5-III |
| GSM923100 |
cells expressing the marker NG2: NG2-I |
| GSM923101 |
cells expressing the marker NG2: NG2-II |
| GSM923102 |
cells expressing the marker NG2: NG2-III |
|
| Relations |
| BioProject |
PRJNA162313 |
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