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| Status |
Public on Apr 27, 2012 |
| Title |
Identification of stage-specific gene expression signatures in response to retinoic acid during the neural differentiation of mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We analyzed gene expression profiling by an bioinformatic approach to identify the stage-specific gene sets associated with neural differentiation of mESCs. We exposed mESCs (B6G-2 cells) to titrated doses of retinoic acid (RA) for 4 days during embryoid body formation and then performed morphological analysis on day of differentiation (DoD) 8 and 29, or genomic microarray analysis on DoD 0, 2, 8 and 29. Microarray data were analyzed by clustering methods such as self-organizing map and principal component analysis. A gene network analysis for selected gene sets was then performed using Bayesian statistics to identify stage-specific gene expression signatures in response to RA.
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| Overall design |
9 total samples: 1 vehcle, 8 Retinoic acid and two or three doses of each developmental stage(Day) [Vehicle Control (0.1% dimethyl sulfoxide), estradiol (1nM and 10nM), dehydrotestosterone (1nM and 10nM) bisphenol A (1pM and 100pM), triiodothyronine (1nM and 10nM), dexamethasone (1nM and 10nM), 2,3,7,8-tetrachlorodibenzo-p-dioxin (1nM and 10nM), 4(OH)-2’3,3’4’-5’-pentachlo biphenyls (1nM and 10nM), thalidomide (100nM and 0.01mM), bis(2-ethylhexyl)phthalate (0.001mM and 0.1mM), permethrin (100nM and 0.01mM), cyclopamine (100nM and 0.01mM), methoprene acid (0.001mM and 0.1mM)]; 1 Timepoint [48 hours].
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| Contributor(s) |
Sone H, Ohsako S |
| Citation missing |
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| Submission date |
Apr 26, 2012 |
| Last update date |
Apr 28, 2012 |
| Contact name |
Sone Hideko |
| E-mail(s) |
hsone@nies.go.jp
|
| Phone |
81298502420
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| Organization name |
National Institute for Environmental Studies
|
| Department |
Center for Environmental Risk Research
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| Lab |
Environmental Exposure Research Section
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| Street address |
onogawa16-2
|
| City |
tsukuba |
| State/province |
ibaraki |
| ZIP/Postal code |
305-8506 |
| Country |
Japan |
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| Platforms (1) |
| GPL6481 |
Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA162391 |
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