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| Status |
Public on Jun 21, 2012 |
| Title |
Genome-wide microRNA expression analysis of clear cell Renal Cell Carcinoma by next generation deep sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
MicroRNAs (miRNAs), non-coding RNAs regulating gene expression, are frequently aberrantly expressed in human cancers. Next-generation deep sequencing technology enables genome-wide expression profiling of known miRNAs and discovery of novel miRNAs at unprecedented quantitative and qualitative accuracy. Deep sequencing was performed on 22 fresh frozen clear cell renal cell carcinoma (ccRCC), 11 non-tumoral renal cortex (NRC) samples and 2 ccRCC cell lines (n=35). The 22 ccRCCs patients belonged to 3 prognostic sub-groups, i.e. Those without disease recurrence, with recurrence and with metastatic disease at diagnosis
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| |
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| Overall design |
Deep sequencing was performed on 22 fresh frozen clear cell renal cell carcinoma (ccRCC), 11 non-tumoral renal cortex (NRC) samples and 2 ccRCC cell lines (n=35). The 22 ccRCCs patients belonged to 3 prognostic sub-groups, i.e. Those without disease recurrence, with recurrence and with metastatic disease at diagnosis.
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| |
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| Contributor(s) |
Osanto S, Qin Y, Buermans HP, Berkers J, Lerut E, Goeman J, van Poppel H |
| Citation(s) |
22745662 |
| Submission date |
Apr 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Henk Buermans |
| E-mail(s) |
h.buermans@lumc.nl
|
| Phone |
+31715268558
|
| Organization name |
Leiden University Medical Center
|
| Department |
Human Genetics
|
| Lab |
Leiden Genome Technology Center
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| State/province |
ZH |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (35)
|
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| Relations |
| BioProject |
PRJNA162397 |
| SRA |
SRP012546 |
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