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Series GSE37635 Query DataSets for GSE37635
Status Public on May 26, 2013
Title Collagen Dynamics and Transcriptional Profiling in Bleomycin-induced Pulmonary Fibrosis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Collagen deposition is a key process during idiopathic pulmonary fibrosis (IPF); however, little is known about the dynamics of collagen formation during disease development. Tissue samples of early stages of human disease are not readily available and it is difficult to identify changes in collagen content, since standard collagen analysis does not distinguish between 'old' and 'new' collagen. Therefore, the current study aimed to (i) investigate the dynamics of new collagen formation in mice using bleomycin-induced lung fibrosis in which newly synthesized collagen was labelled with deuterated water and (ii) use this information to identify genes and processes correlated to new collagen formation from gene expression analysis.
Lung fibrosis was induced in female C57BL/6 mice by bleomycin instillation and sacrificed. Animals were sacrificed at 1 to 5 weeks after fibrosis induction. Collagen synthesized during the week before sacrifice was labelled with deuterium by providing mice with deuterated drinking water. After sacrifice, lung tissue was collected for microarray analysis, determination of new collagen formation, and histology. Deuterated water labelling showed a strong increase in new collagen formation already during the first week after fibrosis induction and a complete return to baseline at five weeks. Correlation of new collagen formation data with gene expression data revealed fibrosis specific processes, of which proliferation was an unexpected one. This was confirmed by measuring cell proliferation and collagen synthesis simultaneously using deuterated water incorporation. Furthermore, new collagen formation strongly correlated with gene expression of e.g. elastin, tenascin C, MMP-14, lysyl oxidase, and type V collagen. These data demonstrate, using a novel combination of technologies, that proliferation and extracellular matrix production are correlated to the core process of fibrosis, i.e. the formation of new collagen. In addition, it identified genes directly correlated to fibrosis, thus providing more insight into the aetiology of IPF.
 
Overall design Total RNA was obtained from mouse lungs at timepoint 0 as a control (n = 7) or timepoints 1 (n = 7), 2 (n = 6), 3 (n = 6), 4 (n = 6) or 5 (n = 6) weeks after bleomycin-instillation to induce lung fibrosis.
 
Contributor(s) Blaauboer ME, Emson C, Verschuren L, van Erk M, Turner S, Everts V, Hanemaaijer R, Stoop R
Citation(s) 23648810
Submission date Apr 27, 2012
Last update date Jun 14, 2018
Contact name Lars Verschuren
E-mail(s) lars.verschuren@tno.nl
Phone +31888666039
Organization name TNO
Department Microbiology and Systems Biology
Street address Utrechtseweg 48
City Zeist
ZIP/Postal code 3704HE
Country Netherlands
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (38)
GSM923470 control_rep1
GSM923471 control_rep2
GSM923472 control_rep3
Relations
BioProject PRJNA162383

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37635_RAW.tar 3.1 Mb (http)(custom) TAR
GSE37635_non-normalized.txt.gz 6.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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