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Series GSE37648 Query DataSets for GSE37648
Status Public on Nov 25, 2013
Title Gene signatures of normal hTERT immortalized ovarian epithelium and fallopian tube epithelium (paired cultures from 2 donor patients)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Most epithelial ovarian cancers are thought to arise from different cells in the ovarian or fallopian tube epithelium. We hypothesized that these distinct cells-of-origin may play a role in determining ovarian tumor phenotype and also could inform the molecular classification of ovarian cancer. To test this hypothesis, we developed new methods to isolate and culture paired normal human ovarian (OV) and fallopian tube (FT) epithelial cells from multiple donors without cancer and identified a cell-of-origin gene expression signature that distinguished these cell types within the same patient. Application of the OV versus FT cell-of-origin gene signature to gene expression profiles of primary ovarian cancers permitted identification of distinct OV and FT-like subgroups among these cancers. Importantly, the normal FT-like tumor classification correlated with a significantly worse disease-free survival. This work describes a new experimental method for culture of normal human OV and FT epithelial cells from the same patient. These findings provide new evidence that cell-of-origin is an important source of ovarian tumor heterogeneity and the associated differences in tumor phenotype.
 
Overall design We analyzed 12 samples from two donor patients and established cultures of both ovarian epithelium and fallopian tube epithelium (hTERT immortalized), each with 3 replicates (different culture passages).
 
Contributor(s) Merritt MA, Ince TA
Citation(s) 24303006
Submission date Apr 29, 2012
Last update date Mar 25, 2019
Contact name Melissa Amy Merritt
Organization name Dana-Farber Cancer Institute
Department Biostatistics and Computational Biology
Lab Computational Biology and Functional Genomics (Quackenbush)
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215-5450
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM925035 patient1_ovary_rep1
GSM925036 patient1_ovary_rep2
GSM925037 patient1_ovary_rep3
Relations
BioProject PRJNA162463

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37648_RAW.tar 67.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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