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| Status |
Public on May 01, 2015 |
| Title |
Granulomatous response to Coxiella burnetii, the agent of Q fever: Activation of type I interferon-related genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The formation of granulomas is associated with the resolution of Q fever, a zoonosis due to Coxiella burnetii; however the molecular mechanisms of granuloma formation remain poorly understood. We generated human granulomas with peripheral blood mononuclear cells and beads coated with C. burnetii, using BCG extracts as controls. A microarray analysis showed dramatic changes in gene expression in granuloma cells compared with peripheral blood mononuclear cells. About 60% of modulated genes were common to C. burnetii and BCG granulomas including M1-related genes. C. burnetii granulomas also expressed a specific transcriptional profile that was essentially enriched in genes associated with type I interferon response (IFI44, IFI44L, IFI6, OAS1, OAS2, OAS3, ISG15, IFIT1, IFITM2, IFITM3, MX1 and MX2). Real-time RT-PCR confirmed that C. burnetii especially increased the expression of interferon-stimulated genes in granulomas generated from controls or Q fever patients. Our results showed that granuloma formation is associated with a core of transcriptional response, but that the granulomatous response to C. burnetii is characterized by the activation of type I interferon-related genes, conferring a new role for type I interferon response in the control of C. burnetii infection.
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| Overall design |
Human Peripheral Blood Mononuclear Cells (PBMC) were co-cultured with beads coated with Mycobacterium bovis strain Bacille Calmette Guerin (BCG) extracts or Coxiella burnetii (C.burnetii) extracts for 8 days and PBMC at day 0 were used as controls. For each condition ( unstimulated, BCG and C.burnetii), each microarray is a replicate of the same biological sample.
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| Contributor(s) |
Faugaret D, Ben Amara A, Delaby A, Lépolard C, Ghigo E, Capo C, Raoult D, Mège J, Textoris J |
| Citation(s) |
25566510 |
| Submission date |
Apr 30, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Julien Textoris |
| E-mail(s) |
julien.textoris@gmail.com
|
| Phone |
+33 472 119 546
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| Organization name |
bioMérieux
|
| Department |
Medical Diagnostic Discovery Department (MD3)
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| Lab |
Joint Research Unit - bioMérieux / HCL
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| Street address |
Hôpital Edouard herriot - Pavillon P; 5 place d'Arsonval
|
| City |
Lyon |
| ZIP/Postal code |
69437 |
| Country |
France |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA162583 |
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