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| Status |
Public on Jul 01, 2012 |
| Title |
Unraveling cell type–specific and reprogrammable human replication origin signatures associated with G-quadruplex consensus motifs |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Short nascent strands purification coupled to next-generation sequencing allowed us to identify replication origins on human genome in an extensive way, by mapping replication origins in 4 different cell types, IMR-90 fibroblasts, hESC H9 cells, iPSC Th Cl-4 cells and HeLa cells.
We demonstrated the existence of a cell type-specific reprogrammable signature of the cell identity revealed by specific efficiencies of conserved origin positions and not by the selection of cell-type specific subsets of origins.
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| Overall design |
4 different cell types were analyzed. For each cell types, 2 different biological replicates of short nascent strands at replication origins were purified. Each SNS sample was sequencing at least one time.
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| Contributor(s) |
Besnard E, Babled A, Lapasset L, Milhavet O, Parrinello H, Dantec C, Marin J, Lemaitre J |
| Citation(s) |
22751019 |
| Submission date |
May 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Emilie Besnard |
| Organization name |
Institute of Functional Genomics
|
| Lab |
Genome Plasticity and Aging
|
| Street address |
141 rue de la Cardonille
|
| City |
MONTPELLIER |
| ZIP/Postal code |
34000 |
| Country |
France |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA163241 |
| SRA |
SRP012667 |
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