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| Status |
Public on May 01, 2013 |
| Title |
RNA-seq in breast cancer cell lines after manipulation of miR-23b expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-23b in both MCF-7 and MDA-MB-231 breast cancer cell lines
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| |
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| Overall design |
Our study implicates miR-23b in cytoskeletal remodeling in breast cancer.
To evaluate the entire set of genes modulated by miR-23b we performed RNA-seq after ectopic manipulation of this miRNA in breast cancer cell lines.
We over-expressed this miRNA in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. RNA-seq analysis revealed a number of candidate targets of this miRNA.
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| |
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| Contributor(s) |
Castellano L, Pellegrino L |
| Citation(s) |
23580553 |
| Submission date |
May 10, 2012 |
| Last update date |
Feb 21, 2023 |
| Contact name |
Leandro Castellano |
| E-mail(s) |
l.castellano@sussex.ac.uk
|
| Organization name |
University of Sussex
|
| Street address |
Falmer, JMS Building
|
| City |
Brighton |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA165157 |
| SRA |
SRP013022 |
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