 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 18, 2012 |
| Title |
Overexpression of Snail is associated with lymph node metastasis and poor prognosis in patients with gastric cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Epithelial-mesenchymal transition (EMT) is in the highlights as a significant role in tumor progression and invasion. Snail was known as the one of regulators of EMT in various malignant tumors. The aim of this study was to investigate the effect of Snail on invasiveness/migratory ability of gastric cancer cell lines and clinicopathological and prognostic significance of Snail over-expression using immunohistochemistry in tissue microarray of 314 gastric adenocarcinomas (GC). Differential gene expression in Snail over-expressed GC was investigated using cDNA microarray analysis. Silencing of Snail by ShRNA induced decreased of invasion, migration of gastric cancer cell lines. In contrast, over-expression of Snail induced increased invasiveness and migratory ability of gastric cancer cell lines in accordance increase of VEGF and MMP11. Furthermore, the over-expression of Snail (≥75% nuclear staining of Snail) was significantly associated with tumor progression (p<0.0001), lymph node metastases (p=0.002), lymphovascular invasion (p=0.002), and perineural invasion (p=0.002) in 314 GC patient. Snail over-expression was also associated with poor prognosis (shorter survival) in GC patients (p=0.023). cDNA microarray revealed 213 differential expressed genes in Snail over-expressed GC tissues, including genes related to metastasis, invasion. Based on above results, it was suggested that Snail plays a significant role in invasiveness/migratory ability of GCs. In addition, Snail might be used to predictive biomarker for evaluation of prognosis or aggressiveness in GCs.
|
| |
|
| Overall design |
48 primary gastric adenocarcinoma fresh frozen tissues were used for microarray. All the tissues were obtained after curative resection after pathologic confirm at Pusan National University Hospital (PNUH, Busan, Korea) and Cheonnam University Hospital (CNUH, Cheonnam, Korea). Microarray experiment and data analysis were done at Cancer research institute, PNUH, Busan, Korea.
|
| |
|
| Contributor(s) |
Park D, Choi C, Jeon T, Kim D, Lee J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 17, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Chae Hwa Kwon |
| E-mail(s) |
chkwon@pusan.ac.kr
|
| Organization name |
Cancer Research Institue, Pusan National University
|
| Street address |
1-10 Ami-Dong, Seo-Gu
|
| City |
Busan |
| ZIP/Postal code |
602-739 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (48)
|
|
| Relations |
| BioProject |
PRJNA167122 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38024_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE38024_non-normalized.txt.gz |
13.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...