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Series GSE38036 Query DataSets for GSE38036
Status Public on Jun 16, 2012
Title Fry et al Drosophila Ethanol Resistance Selection Experiment
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Outbred D.melanogaster populations subjected to >300 generations of natural selection on either control, or 12% ethanol, or variable food (2 replicates each) and exposed, as first instar larvae, to either water control or 12% ethanol.
 
Overall design Six experimental populations were established from an outbred base population derived from flies collected in Raleigh, NC, U.S.A. in 1995. They were selected in either control (R), ethanol (E) or variable (M) selection regimes (2 replicate populations in each) according to the following protocol. Each generation of each population was initiated by allowing 20-30 adults to lay eggs in each of 50 vials containing either standard cornmeal-molasses-agar medium (all the vials in the R populations, and half of the vials in the M populations) or medium supplemented with 12% ethanol (half of the vials in the M populations, and all of the vials in the E populations). After 14 days, progeny from the different vials are mixed before being used to set up the next generation. Gene expression experiment conducted after >300 generations of selection as described below.
 
Contributor(s) Fry JD, Yampolsky LY, Glazko GV
Citation(s) 22774776
Submission date May 17, 2012
Last update date Aug 28, 2018
Contact name Lev Yampolsky
E-mail(s) yampolsk@etsu.edu
Phone 423-439-4359
Fax 423-439-5958
URL http://faculty.etsu.edu/yampolsk
Organization name East Tennessee State University
Department Biological Sciences
Street address ETSU
City Johnson City
State/province TN
ZIP/Postal code 37614
Country USA
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (34)
GSM932813 D.melanogaster_selection=R1_exposure=E1
GSM932814 D.melanogaster_selection=R1_exposure=W1
GSM932815 D.melanogaster_selection=M1_exposure=E1
Relations
BioProject PRJNA167088

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38036_RAW.tar 74.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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