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| Status |
Public on Oct 01, 2012 |
| Title |
Murine Testes: Germ Cell-Specific Sin3a Knockout vs. Hemizygous vs. Wild Type at Postnatal Day 0 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chromatin modifier Swi-independent 3a (SIN3A), together with associated histone deacetylases, influences gene expression during development and differentiation through multiple transcription factors in a cell-specific manner. Sin3a is essential for the maintenance of inner cell mass cells of mouse blastocysts, embryonic fibroblasts, and myoblasts, but is not required for the survival of trophectoderm or Sertoli cells. To better understand how this transcriptional regulator modulates cells at different developmental stages within a single lineage, we used conditional gene targeting in mice to ablate Sin3a from perinatal quiescent male gonocytes and from postnatal differentiating spermatogonia.
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| Overall design |
Gene expression in the whole testes of age-matched Ddx4(Vasa/Mvh)-cre mediated conditional Sin3a gene-targeted(VSKO) mice, germ cell-specific Sin3a hemizygous (HEMI) mice, and wild type (WT) mice was measured at postnatal day 0. Two biological replicate animals were used for each condition.
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| Contributor(s) |
Payne CJ, Gallagher SJ, Kofman AE, Dannenberg J, DePinho RA, Braun RE |
| Citation(s) |
23085237 |
| Submission date |
May 21, 2012 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Christopher J. Payne |
| E-mail(s) |
c-payne@northwestern.edu
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Street address |
2430 N. Halsted St.
|
| City |
Chicago |
| ZIP/Postal code |
60614 |
| Country |
USA |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (6)
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| GSM934572 |
Testes_Germ Cell-Specific Sin3a KO_rep 1 |
| GSM934573 |
Testes_Germ Cell-Specific Sin3a KO_rep 2 |
| GSM934574 |
Testes_Germ Cell-Specific Sin3a Hemizygous_rep 1 |
| GSM934575 |
Testes_Germ Cell-Specific Sin3a Hemizygous_rep 2 |
| GSM934576 |
Testes_Sin3a Wild Type_rep 1 |
| GSM934577 |
Testes_Sin3a Wild Type_rep 2 |
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| Relations |
| BioProject |
PRJNA167202 |
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