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Series GSE38173 Query DataSets for GSE38173
Status Public on Jan 03, 2013
Title The Histone H2B Monoubiquitination Regulatory Pathway is Required for Differentiation of Multipotent Stem Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Extensive changes in post-translational histone modifications accompany the rewiring of the transcriptional program during stem cell differentiation. However, the mechanisms controlling the changes in specific chromatin modifications and their function during differentiation remain only poorly understood. We show that histone H2B monoubiquitination (H2Bub1) significantly increases during differentiation of human mesenchymal stem cells (hMSCs), various lineage-committed precursor cells and in diverse organisms. Furthermore, the H2B ubiquitin ligase RNF40 is required for the induction of differentiation markers and transcriptional reprogramming of hMSC. This function is dependent upon CDK9 and the WAC adaptor protein, which are required for H2B monoubiquitination. Finally, we show that RNF40 is required for the resolution of the H3K4me3/H3K27me3 bivalent poised state on lineage-specific genes during the transition from an inactive to active chromatin conformation. Thus, these data indicate that H2Bub1 is required for maintaining multipotency of hMSC cells and plays a central role in controlling stem cell differentiation.
 
Overall design This set contains 29 microarray samples and includes the following 5 conditions: undifferentiated hMSCs, 2 day osteoblast differentiation, 5 day osteoblast differentiation, 2 day adipocyte differentiation, and 5 day adipocyte differentiation. 3 siRNA control samples and 3 RNF40 knockdown samples for each condition (except two control siRNA samples for 2 days osteoblast differentiation).
 
Contributor(s) Karpiuk O, Najafova Z, Kramer F, Hennion M, Galonska C, König A, Snaidero N, Vogel T, Shchebet A, Begus-Nahrmann Y, Kassem M, Simons M, Shcherbata H, Beissbarth T, Johnsen SA
Citation(s) 22681891
Submission date May 23, 2012
Last update date Aug 13, 2018
Contact name Frank Kramer
E-mail(s) frank.kramer@med.uni-goettingen.de
Organization name University Medical Center Göttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (29)
GSM936228 Control siRNA - Undifferentiated, rep1
GSM936229 Control siRNA - Undifferentiated, rep2
GSM936230 Control siRNA - Undifferentiated, rep3
Relations
BioProject PRJNA167361

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38173_RAW.tar 26.2 Mb (http)(custom) TAR
GSE38173_non-normalized.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table
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