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Series GSE3822 Query DataSets for GSE3822
Status Public on May 01, 2006
Title Complete embryonic E11.5 metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. Targets from multiple biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430_v2 arrays.
Keywords: embryonic metanephric kidney, kidney development, Hoxa11, Hoxd11, compound null targeted mice
Overall design Using microdissection techniques, ureteric bud and metanephric mesenchyme were dissected from E11.5 kidney rudiments allowing the identificated genes specifically regulated in either structure. In addition, Hoxa11, Hoxd11 compound null E11.5 metanephric mesenchyme were normalized to wild type embryonic controls allowing the identification of potential Hox targets in normal kidney development. Each structure/genotype were represented in biological (seperate embryo) replicate.
Contributor(s) Schwab K, Potter SS
Citation(s) 16581055
Submission date Dec 14, 2005
Last update date Feb 11, 2019
Contact name Kristopher Robert Schwab
Organization name Cincinnati Children's Hospital Research Foundation
Department Developmental Biology
Lab S. Steven Potter
Street address 3333 Burnett Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM87677 HoxA11, D11 double null embryonic E11.5 Metanephric Mesenchyme replicate 1
GSM87678 Wild type embryonic E11.5 Metanephric Mesenchyme replicate 5
GSM87679 Wild type embryonic E11.5 Ureteric Bud replicate 1
BioProject PRJNA94039

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Supplementary file Size Download File type/resource
GSE3822_RAW.tar 93.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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