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| Status |
Public on Apr 25, 2014 |
| Title |
mRNA expression in iPS cells generated by a synthetic self-replicative RNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated iPS cells with a synthetic self-replicative RNA that expresses four independent reprogramming factors (OCT4, KLF4, SOX2 and either c-MYC or GLIS1). We performed whole genome RNA sequencing (RNA-seq) of iPS cell clones, parental BJ and HUES9 ES cell controls. All iPS cell clones analyzed by RNA-seq showed unsupervised hierarchical clustering and expression signatures characteristic of human HUES9 ES cells that were highly divergent from parental human fibroblasts.
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| |
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| Overall design |
RNA-seq in two OKS-iM iPS clones (generated from OCT4, KLF4, SOX2 and cMYC expressing RNA replicon), two OKS-iG clones (generated from OCT4, KLF4, SOX2 and GLIS1 expressing RNA replicon), HUES9 and BJ cells.
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| |
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| Contributor(s) |
Li H, Yoshioka N, Fu X, Dowdy S |
| Citation(s) |
23910086 |
| Submission date |
May 25, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Hai-Ri Li |
| E-mail(s) |
hairili@ucsd.edu
|
| Phone |
858-534-4926
|
| Organization name |
University of California, San Diego
|
| Department |
Medicine
|
| Lab |
Dr. Francesca Telese
|
| Street address |
9500 Gilman Dr.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| SRA |
SRP013402 |
| BioProject |
PRJNA167504 |
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